Search Results for: Gut Microbiota
Safety evaluation of neo transgenic pigs by studying changes in gut microbiota using high-throughput sequencing technology
Abstract: The neo (neomycin phosphotransferase) gene is widely used as a selection marker in the production of genetically engineered animals and plants. Recent attention has been focused on safety concerns regarding neo transgene expression. In this study, neo transgenic and non-transgenic piglets were randomly assigned into Group A and Group B to evaluate effects of …
Effects of Roundup (glyphosate) on gut microorganisms of farm animals
Abstract: Glyphosate, is a broad-spectrum, nonselective, systemic herbicide, existing in the form of the acid itself or formulated as a salt. Roundup consists of the glyphosate isopropylamine (IPA) salt, water and a surfactant, often a polyethoxylated tallowamine, POEA. Glyphosate inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in the shikimate pathway, which leads to formation of aromatic amino acids. …
Sex-dependent impact of Roundup on the rat gut microbiome
Abstract: A growing body of research suggests that dysbiosis of the gut microbiota induced by environmental pollutants, such as pesticides, could have a role in the development of metabolic disorders. We have examined the long-term effects of 3 doses of the Roundup(R) herbicide (made of glyphosate and formulants) on the gut microbiota in male and female …
Glyphosate based-herbicide exposure affects gut microbiota, anxiety and depression-like behaviors in mice
Abstract: Recently, a number of studies have demonstrated the profound relationship between gut microbiota (GM) alterations and behavioral changes. Glyphosate-based herbicides (GBH) have been shown to induce behavioral impairments, and it is possible that they mediate the effects through an altered GM. In this study, we investigated the toxic effects of GBH on GM and …
Persistence of DNA studied in different ex vivo and in vivo rat models simulating the human gut situation
Abstract: This study aimed to evaluate the possibility of DNA sequences from genetically modified plants to persist in the gastrointestinal (GI) tract. PCR analysis and transformation assays were used to study DNA persistence and integrity in various ex vivo and in vivo systems using gnotobiotic rats. DNA studied was either plasmid DNA, naked plant DNA …
The Ramazzini Institute 13-week pilot study on glyphosate and Roundup administered at human-equivalent dose to Sprague Dawley rats: Effects on the microbiome
Abstract: Background Glyphosate-based herbicides (GBHs) are broad-spectrum herbicides that act on the shikimate pathway in bacteria, fungi, and plants. The possible effects of GBHs on human health are the subject of an intense public debate for both its potential carcinogenic and non-carcinogenic effects, including its effects on microbiome. The present pilot study examines whether exposure …
The effect of glyphosate on potential pathogens and beneficial members of poultry microbiota in vitro
Abstract: The use of glyphosate modifies the environment which stresses the living microorganisms. The aim of the present study was to determine the real impact of glyphosate on potential pathogens and beneficial members of poultry microbiota in vitro. The presented results evidence that the highly pathogenic bacteria as Salmonella Entritidis, Salmonella Gallinarum, Salmonella Typhimurium, Clostridium …
