Search Results for: Mice

Abstract: The conclusion of a 28-day study feeding mice double-stranded (ds)RNA published in this journal was that: “toxicity and target gene expression as surrogate measures of any biologically meaningful absorption/biodistribution or activity of ingested dsRNA. This is because any relevant impact of an absorbed nucleic acid on gene expression (e.g., ability to reach a target …

Abstract: An organism of which gene sequence is modified or a new feature is added to it is called Genetically Modified Organism (GMO). This study researches the potential physiologic and genotoxic effects of a genetically modified organism, maize (Zea mays L.) on Mice. While physiologic effects are identified by measuring live-born weight, live weight, organ …

Abstract: This study evaluated the gut and peripheral immune response to genetically modified (GM) maize in mice in vulnerable conditions. Weaning and old mice were fed a diet containing MON810 or its parental control maize or a pellet diet containing a GM-free maize for 30 and 90 days. The immunophenotype of intestinal intraepithelial, spleen, and …

Abstract: BACKGROUND AND AIM. To explore the glyphosate-induced mutagenesis in mice. MATERIALS AND METHODS. In micronucleus test(MNT), glyphosate was given twice via intragastric administration to different groups of mice at different concentrations(2 320 mg/kg, 1 160 mg/kg, 580 mg/kg) at a 24-hour interval. Mice were killed 6 hours after last treatment to determine the micronucleus …

Abstract: Formulated and sporulated cultures of Bacillus thuringiensis (Bt) have been widely used against insect pests, but after the advent of genetically modified plants expressing δ-endotoxins, the bioavailability of Cry proteins has been increased. For biosafety reasons their adverse effects should be studied, mainly for non-target organisms. Thus, we evaluated, in Swiss albino mice, the …

Abstract: The use of genetically modified organisms (GMO) in dairy products requires evaluation of the DNA transfer capacity from such organisms among the human intestinal microflora. Thus, both in vitro and in vivo [in the digestive tract (DT) of mice] transfer from Lactococcus lactis donor strains of the conjugative plasmid pIL205 (CmR) and the non-conjugative …

Abstract: Recently, a number of studies have demonstrated the profound relationship between gut microbiota (GM) alterations and behavioral changes. Glyphosate-based herbicides (GBH) have been shown to induce behavioral impairments, and it is possible that they mediate the effects through an altered GM. In this study, we investigated the toxic effects of GBH on GM and …

Abstract: Recently we discovered that the Cry1Ac protoxin of Bacillus thuringiensis administered to Balb/c mice intraperitoneally (i.p.) or intragastrically is a systemic and intestinal immunogen as potent as cholera toxin. To further characterize the mucosal immunogenicity of Cry1Ac we additionally tried the intranasal (i.n.) and rectal routes and used enzyme-linked immunoassays to determine anti-Cry1Ac antibody …

Abstract: The insecticidal properties of Cry-endotoxins from Bacillus thuringiensis (Bt) have long been used as spore-crystals in commercial spray formulations for insect control. Recently, some Bt-endotoxin genes have been cloned in many different plants. Toxicological evaluations of three spore-crystal endotoxins, BtCry1Ia, BtCry10Aa and BtCry1Ba6 from B. thuringiensis, were carried out on mice to understand their …

Abstract: Food-ingested foreign DNA is not completely degraded in the gastrointestinal tract of mice. Phage M13mp18 DNA as a test molecule devoid of homology to mouse DNA was pipette-fed to or added to the food supply of mice. The fate of this foreign DNA in the animals was followed by several methods. In 84 animals, …

Abstract: Genetically modified (GM) soybean, resistant to the pesticide Round up, is a widely spread crop for animal and human consumption. We have focused our attention on embryos at 4-8 cells stage from mice fed on a diet containing 14% of GM soybean, analysing the possible alterations in transcription and splicing processes. We have evaluated, …

Abstract: Glufosinate ammonium (GLA) is one of the most widely used herbicides in agriculture. As is the case for most pesticides, potential adverse effects of GLA have not been studied from the perspective of developmental neurotoxicity. Early pesticides exposure may weaken the basic structure of the developing brain and cause permanent changes leading to a …

Abstract: Roundup® is extensively used for weed control worldwide. Residues of this compound may lead to side effects of the male reproductive system. However, the toxic effects and mechanisms of Roundup® of male germ cells remain unclear. We aimed to investigate the apoptosis-inducing effects of Roundup® on mouse male germ cells and explore the role …

Abstract: The aim of this study was to evaluate the possible hepatorenal toxicity and genotoxicity from eating meat meal of genetically modified (GM) Japanese quail in 90 days dietary experiment using micronucleus (MN) test, mitotic index (MI) and RAPD-PCR for genotoxicity assay, ALT, AST, urea and creatinine for hepatorenal toxicity. Four groups of Swiss male …

Abstract: Glyphosate is a broad spectrum herbicide. Fruits and vegetables improve health and a diminish chronic degenerative processes. This study aims to investigate orange juice protective role against the toxicity of glyphosate induced in mice. Fifty six adult male albino mice divided into seven groups. Oral administration of glyphosate caused a significant increase in MDA …

Abstract: Glyphosate (N-(phosphonomethyl) glycine, P), a herbicide, used to control unwanted annual and perennial plants all over the world. Nevertheless, occupational and environmental exposure to pesticides can pose a threat to nontarget species including human beings. Therefore, in the present study, genotoxic effects of the herbicide glyphosate were analyzed by measuring chromosomal aberrations (CAs) and …

Abstract: Bacillus thuringiensis (Bt) has been widely used in foliar sprays as part of integrated pest management strategies against insect pests of agricultural crops. Since the advent of genetically modified plants expressing Bt δ‐endotoxins, the bioavailability of Cry proteins has increased, and therefore for biosafety reasons their adverse effects should be studied, mainly for nontarget …

Abstract: Excessive amounts of the herbicide glyphosate are incorporated daily to the soil and the ecosystems. AMPA is its major environmental breakdown product. In this study we determined the levels of thiobarbituric acid reactive substances (TBARs); quantified superoxide dismutase (SOD) and catalase (CAT) activity in liver, kidney, lung and heart, and performed the comet assay …

Abstract: The problems of the introduction of transgenic products into the consumer market were examined. The impact of the genetically modified potatoes on the cytological and morphological parameters in mice was investigated. An increase in body mass and in hearts mass and a decrease in spleens mass, degeneration of the ovaries and changing of the …

Abstract: The article is devoted to the introduction of transgenic products into the consumer market. The impact of the genetically modified potatoes on the cytological and morphological parameters of mice was investigated. Transgenic potato plants were produced by agrobacterium-mediated transformation using genetic constructions 35S-proSmAMP with marker gene npt11 of the resistance to the kanamycin. An increase in hearts …

Abstract: We carried out ultrastructural morphometrical and immunocytochemical analyses on pancreatic acinar cell nuclei from mice fed on genetically modified (GM) soybean, in order to investigate possible structural and molecular modifications of nucleoplasmic and nucleolar constituents. We found a significant lowering of nucleoplasmic and nucleolar splicing factors as well as a perichromatin granule accumulation in …

Abstract: We evaluated the toxicity of hepatic, hematological, and oxidative effects of glyphosate-Roundup® on male and female albino Swiss mice. The animals were treated orally with either 50 or 500 mg/kg body weight of the herbicide, on a daily basis for a period of 15 days. Distilled water was used as control treatment. Samples of …

Abstract: Glyphosate (N-(phosphonomethyl) glycine, C3 H8 NO5P) is the world’s most widely used herbicide. Nevertheless their benefits, occupational and environmental exposure to pesticides can pose a threat to non-target species including human beings. Numerous laboratory studies have shown that Glyphosate and the roundup formulation can be genotoxic and endocrine disrupting. Therefore, the present study was …