GMO Research
Abstract: An organism of which gene sequence is modified or a new feature is added to it is called Genetically Modified Organism (GMO). This study researches the potential physiologic and genotoxic effects of a genetically modified organism, maize (Zea mays L.) on Mice. While physiologic effects are identified by measuring live-born weight, live weight, organ …
Abstract: The conclusion of a 28-day study feeding mice double-stranded (ds)RNA published in this journal was that: “toxicity and target gene expression as surrogate measures of any biologically meaningful absorption/biodistribution or activity of ingested dsRNA. This is because any relevant impact of an absorbed nucleic acid on gene expression (e.g., ability to reach a target …
Abstract: No direct evidence that genetically modified (GM) food may represent a possible danger for health has been reported so far; however, the scientific literature in this field is still quite poor. Therefore, we carried out an ultrastructural morphometrical and immunocytochemical study on hepatocytes from mice fed on GM soybean, in order to investigate eventual …
Abstract: This study evaluated the gut and peripheral immune response to genetically modified (GM) maize in mice in vulnerable conditions. Weaning and old mice were fed a diet containing MON810 or its parental control maize or a pellet diet containing a GM-free maize for 30 and 90 days. The immunophenotype of intestinal intraepithelial, spleen, and …
Abstract: Consumption by mice of genetically modified soya worsens the general condition in females, them aggression to the posterity amplifies, the parent instinct is lost. At little mice of the first generation are registered: exophtalmos, hyperactivity, paralyses, hydro-nephrosis. At little mice of the first and second generation accumulation of superfluous alive weight of a body …
Abstract: BACKGROUND AND AIM. To explore the glyphosate-induced mutagenesis in mice. MATERIALS AND METHODS. In micronucleus test(MNT), glyphosate was given twice via intragastric administration to different groups of mice at different concentrations(2 320 mg/kg, 1 160 mg/kg, 580 mg/kg) at a 24-hour interval. Mice were killed 6 hours after last treatment to determine the micronucleus …
Abstract: Liver represents a suitable model for monitoring the effects of a diet, due to its key role in controlling the whole metabolism. Although no direct evidence has been reported so far that genetically modified (GM) food may affect health, previous studies on hepatocytes from young female mice fed on GM soybean demonstrated nuclear modifications …
Abstract: In the literature, the reports on the effects of a genetically modified (GM) diet are scanty and heterogeneous; in particular, no direct evidence has so far been reported that GM food may affect human or animal health. Hepatocytes represent a suitable model for monitoring the effects of a GM diet, the liver potentially being …
Abstract: The safety assessment of genetically modified (GM) food and feed is performed to identify the possible effects upon animal and human health, also the long-term, multigenerational influence upon functioning of different organs and systems, such as the immune system. In this study C57BL/6J mice were fed for five consecutive generations with pellets containing 20% …
Abstract: Formulated and sporulated cultures of Bacillus thuringiensis (Bt) have been widely used against insect pests, but after the advent of genetically modified plants expressing δ-endotoxins, the bioavailability of Cry proteins has been increased. For biosafety reasons their adverse effects should be studied, mainly for non-target organisms. Thus, we evaluated, in Swiss albino mice, the …
Abstract: Through feeding Kunming white mice GM feed, study the effect of GM feed to mice reproduction system. Upon feeding one month, matched couples of male and female mice with matured reproduction system are arranged into respective cages. The first time arranged 4 matched groups, i.e. control, treatment group GMI, GMII and GMIII. The control group …
Abstract: The use of genetically modified organisms (GMO) in dairy products requires evaluation of the DNA transfer capacity from such organisms among the human intestinal microflora. Thus, both in vitro and in vivo [in the digestive tract (DT) of mice] transfer from Lactococcus lactis donor strains of the conjugative plasmid pIL205 (CmR) and the non-conjugative …
Abstract: Recently, a number of studies have demonstrated the profound relationship between gut microbiota (GM) alterations and behavioral changes. Glyphosate-based herbicides (GBH) have been shown to induce behavioral impairments, and it is possible that they mediate the effects through an altered GM. In this study, we investigated the toxic effects of GBH on GM and …
Abstract: Recently we discovered that the Cry1Ac protoxin of Bacillus thuringiensis administered to Balb/c mice intraperitoneally (i.p.) or intragastrically is a systemic and intestinal immunogen as potent as cholera toxin. To further characterize the mucosal immunogenicity of Cry1Ac we additionally tried the intranasal (i.n.) and rectal routes and used enzyme-linked immunoassays to determine anti-Cry1Ac antibody …
Abstract: The insecticidal properties of Cry-endotoxins from Bacillus thuringiensis (Bt) have long been used as spore-crystals in commercial spray formulations for insect control. Recently, some Bt-endotoxin genes have been cloned in many different plants. Toxicological evaluations of three spore-crystal endotoxins, BtCry1Ia, BtCry10Aa and BtCry1Ba6 from B. thuringiensis, were carried out on mice to understand their …
Abstract: We have previously shown that, when administered orally to mice, bacteriophage M13 DNA, as a paradigm foreign DNA without homology to the mouse genome, can persist in fragmented form in the gastrointestinal tract, penetrate the intestinal wall, and reach the nuclei of leukocytes, spleen and liver cells. Similar results were obtained when a plasmid …
Abstract: Food-ingested foreign DNA is not completely degraded in the gastrointestinal tract of mice. Phage M13mp18 DNA as a test molecule devoid of homology to mouse DNA was pipette-fed to or added to the food supply of mice. The fate of this foreign DNA in the animals was followed by several methods. In 84 animals, …
Abstract: Genetically modified (GM) soybean, resistant to the pesticide Round up, is a widely spread crop for animal and human consumption. We have focused our attention on embryos at 4-8 cells stage from mice fed on a diet containing 14% of GM soybean, analysing the possible alterations in transcription and splicing processes. We have evaluated, …
Abstract: We have considered the possible effects of a diet containing genetically modified (GM) soybean on mouse testis. This organ, in fact, is a well known bioindicator and it has already been utilized, for instance, to monitor pollution by heavy metals. In this preliminary study, we have focussed our attention on Sertoli cells, spermatogonia and …
Abstract: Glufosinate ammonium (GLA) is one of the most widely used herbicides in agriculture. As is the case for most pesticides, potential adverse effects of GLA have not been studied from the perspective of developmental neurotoxicity. Early pesticides exposure may weaken the basic structure of the developing brain and cause permanent changes leading to a …
Abstract: Roundup® is extensively used for weed control worldwide. Residues of this compound may lead to side effects of the male reproductive system. However, the toxic effects and mechanisms of Roundup® of male germ cells remain unclear. We aimed to investigate the apoptosis-inducing effects of Roundup® on mouse male germ cells and explore the role …
Abstract: The aim of this study was to evaluate the possible hepatorenal toxicity and genotoxicity from eating meat meal of genetically modified (GM) Japanese quail in 90 days dietary experiment using micronucleus (MN) test, mitotic index (MI) and RAPD-PCR for genotoxicity assay, ALT, AST, urea and creatinine for hepatorenal toxicity. Four groups of Swiss male …
Abstract: Glyphosate is a broad spectrum herbicide. Fruits and vegetables improve health and a diminish chronic degenerative processes. This study aims to investigate orange juice protective role against the toxicity of glyphosate induced in mice. Fifty six adult male albino mice divided into seven groups. Oral administration of glyphosate caused a significant increase in MDA …
Abstract: Glyphosate (N-(phosphonomethyl) glycine, C3 H8 NO5P) is the world’s most widely used herbicide. Nevertheless their benefits, occupational and environmental exposure to pesticides can pose a threat to non-target species including human beings. Numerous laboratory studies have shown that Glyphosate and the roundup formulation can be genotoxic and endocrine disrupting. Therefore, the present study was …
Abstract: The aim of the study was to examine effects of the stacked GM crop NK603 x MON810 in different models of long term feeding studies. So far no negative effects of GM corn varieties have been reported in peer-reviewed publications. But the hypothesis, that effects after long term exposure might become evident in multi-generation …
