GMO Research
Abstract: Transformed plants expressing the 3′ two-thirds of the cowpea chlorotic mottle virus (CCMV) capsid gene were inoculated with a CCMV deletion mutant lacking the 3′ one-third of the capsid gene. Although the deletion inoculum replicates in inoculated cells, systemic infections occur only if recombination restores a functional capsid gene. Four of 125 inoculated transgenic …
Abstract: Nicotiana benthamiana was transformed with three different constructs (pCRA1, pCRA2 and pJC1) containing the coat protein coding sequence of African cassava mosaic virus (ACMV). Transformed plants were inoculated with a coat protein deletion mutant of ACMV that induces mild systemic symptoms in control plants. Several inoculated plants of transgenic lines CRA1/3, CRA1/4, CRA2/1 and …
Abstract: An increasing number of animal and plant viruses have been shown to undergo RNA-RNA recombination, which is defined as the exchange of genetic information between nonsegmented RNAs. Only some of these viruses have been shown to undergo recombination in experimental infection of tissue culture, animals, and plants. However, a survey of viral RNA structure …
Cultivos transgénicos: ¨hacia dónde vamos? Algunos efectos sobre el ambiente, la sociedad y la economía de la nueva “recombinación” tecnológica Analiza la evolución y los cambios tecnológicos ocurridos en el cultivo de soja, sus características y efectos ambientales; introduce en las técnicas de mejoramiento genético y en el nuevo conocimeinto de la biotecnología, describiendo sus …
Abstract: Transgene integration into plant genomes is a complex process accompanied by molecular rearrangements. Classic methods that are normally used to study transgenic population genetics are generally inadequate for assessing such integration. Two major characteristics of transgenic populations are that a transgenic genome may harbor many copies of the transgene and that molecular rearrangements can …
Abstract: Intermolecular reconstitution of a plant virus has been detected in whole plants in a system using a defective cauliflower mosaic virus genome and transgenic host plants containing the missing viral gene. The information for the gene VI protein of the virus was integrated into the chromosome of host Brassica napus plants and leaves of …
To the editor: In your account (January 2000) of our prepublication manuscript1, you quote the criticisms but ignore completely our full rebuttal, which was posted on the web last November2. Our manuscript3 reviews and synthesizes the scientific literature on the 35S promoter of the cauliflower mosaic virus (CaMV), used to give constitutive overexpression of transgenes …
Abstract: We have shown previously that gene VI of cauliflower mosaic virus (CaMV) strain D4 governs systemic infection of Nicotiana bigelovii and that transgenic N. bigelovii expressing the D4 gene VI product can complement at least one CaMV isolate for long-distance transport. We have now found that DNA of two other isolates of CaMV recombine …
Abstract: Stable inheritance of the transgene, consistent expression and competitive agronomic properties of transgenic crops are important parameters for successful use of the latter. These properties have been analyzed with 18 homozygous transgenic barley lines of the cultivar Golden Promise. The lines originated from three independent primary transformants obtained by the biolistic method with three …
Abstract: Genetic transformation of plants often results in multiple copies of the introduced DNA at a single locus. To ensure that only a single copy of a foreign gene resides in the plant genome, we used a strategy based on site-specific recombination. The transformation vector consists of a transgene flanked by recombination sites in an …
Text: What’s unspeakable in horizontal gene transfer? Horizontal gene transfer is the transfer of genes across species including those in different kingdoms. It goes counter to both modern genetics and the theory of evolution. Disappointingly, little of the momentous significance of the process comes across in a volume replete with detailed examples. GM foods – …
Citation: Lommel, A. and Xiong, Z., 1991. Reconstitution of a functional red clover necrotic mosaic virus by recombinational rescue of the cell-to-cell movement gene expressed in a transgenic plant. Journal of Cellular Biochemistry A, 15, p.151.
Abstract: Purpose Feral oilseed rape has become widespread in Europe on waysides and waste ground. Its potential as a source of GM impurity in oilseed rape harvests is quantified, for the first time, by a consistent analysis applied over a wide range of study areas in Europe. Methods The maximum contribution of feral oilseed rape …
Text: We would like to address the suggestion of Matzke et al. (1) and others that pararetroviral promoters such as the CaMV 35S are not exotic to plant genomes as they already contain many integrated pararetroviral sequences. The crucial question is whether the CaMV 35S promoter in transgenic constructs poses special risks. We would like …
Abstract: According to the 1996 WHO Report, the world is heading for a major crisis in public health as outbreaks of new and re-emerging infectious diseases are striking at increasing frequencies within the past 10 to 15 years. The current strains of pathogens are moreover, resistant to known treatments; some strains being resistant to all …
Abstract: A new concept for viral oncogenesis is presented which is based on experimental work on the chromosomal integration of adenovirus DNA into mammalian genomes. The mechanism of adenovirus DNA integration is akin to non-sequence-specific insertional recombination in which patch homologies between the recombination partners are frequently observed. This reaction has been imitated in a …
Abstract: The Biotechnology and Food Safety Report issued jointly by the Food and Agriculture Organization (FAO) and World Health Organization (WHO), is the result of an Expert Consultation held in Rome, in October 1996. The Consultation was the latest, possibly the most significant, attempt to reach international agreement on the safety of genetically engineered (GE) …
Abstract: Integration of transgenic DNA into the plant genome was investigated in 13 transgenic oat (Avena sativa L.) lines produced using microprojectile bombardment with one or two cotransformed plasmids. In all transformation events, the transgenic DNA integrated into the plant genome consisted of intact transgene copies that were accompanied by multiple, rearranged, and/or truncated transgene …
Abstract: A substantial literature exists characterizing transgene locus structure from plants transformed via Agrobacterium and direct DNA delivery. However, there is little comprehensive sequence analysis of transgene loci available, especially from plants transformed by direct delivery methods. The goal of this study was to completely sequence transgene loci from two oat lines transformed via microprojectile …
Abstract: This essay discusses two statements: a) there are no fundamental differences between classical genetic improvement (CGI) or breeding and genetic engineering improvement (GEI); the latter is an extension of the first but is more accurate so, it gives greater safety to the consumer; and b) transformation by genetic engineering is equivalent to a natural …
To the editor: Your May 29 editorial1 about the health risks of genetically modified (GM) food was apposite and addresses some of the important medical issues vigorously. It beggars belief that “badly designed, poorly carried out, inaccurately interpreted experiments” could have perpetuated such profound public debate for almost a year. Regrettably, among the correspondents who …
Abstract: Gene targeting (GT) is a technique used to modify endogenous genes in target genomes precisely via homologous recombination (HR). Although GT plants are produced using genetic transformation techniques, if the difference between the endogenous and the modified gene is limited to point mutations, GT crops can be considered equivalent to non-genetically modified mutant crops …
Abstract: Transgenic potato plants with the nptII gene coding for neomycin phosphotransferase (kanamycin resistance) as a selection marker were examined for the spread of recombinant DNA into the environment. We used the recombinant fusion of nptII with the tg4 terminator for a novel biomonitoring technique. This depended on natural transformation of Acinetobacter sp. strain BD413 …
