Search Results for: Transgenic

Abstract: We compared metabolic changes in transgenic maize lines (differing in genetic backgrounds and exogenous genes insertion) with non-transgenic counterparts. At the corn seedling stage, entirely expanding leaves were obtained from maize lines 1 (transgenic maize SK12-5; overexpressing cry1Ab/cry2Aj; parental maize ZD958), 2 (transgenic maize IE034; overexpressing cry1Ie; parental maize ZD958), 3 (transgenic maize Bt799; …

Abstract: Antibiotic resistance rates have increased in both clinical and environmental bacteria over the past several decades. While the causes underlying these trends have been investigated in a clinical setting, little work has been done to estimate the contribution of antibiotic resistance genes (ARGs) derived from non-traditional sources like transgenic (GM) crops. The cultivation and …

Abstract: Laser ablation inductively coupled plasma mass spectrometry is used for determining Fe in leaves of transgenic (variety M 7211RR) and non-transgenic (variety MSOY 8200) soybean, grown for 21 days in a growth chamber at controlled temperature (27 ± 0.1 °C) and photoperiod (12 h). The same technique, but in the imaging mode, is also …

Abstract: The biochemical properties of soil have often been described as early and sensitive indicators of ecological changes in both natural soil and agroecosystem. In the current study, the impacts of the amendment of Bt-transgenic rice (KMD) straw on biological activities in water-flooded soil were investigated under laboratory conditions and compared with non-transgenic rice (Xiushui …

Abstract: Concern has been often expressed regarding the impact and persistence of transgenes that enter wild populations via gene flow. The impact of a transgene and its persistence are largely determined by the relative fitness of transgenic hybrids and hybrid derivatives compared to non-transgenic plants. Nevertheless, few studies have addressed this question experimentally in the …

Abstract: Effects of pesticide applications, based on an IPM program on cotton bollworm, Helicoverpa armigera Hübner, cotton mirids and cotton leafhoppers, were evaluated in transgenic Bt-cotton and non-transgenic cotton agroecosystems between 1999 and 2001 in China. Differences in pest populations between cotton varieties were also compared. In 1999 and 2000, bollworm populations on non-transgenic cotton …

Abstract: RNA interference (RNAi) is considered as a potential modality for clinical treatment and anti-virus animal breeding. Here, we investigate the feasibility of inhibiting classical swine fever virus (CSFV) replication by short hairpin RNA (shRNA) in vitro and in vivo. We generate four different shRNA-positive clonal cells and two types of shRNA-transgenic pigs. CSFV could …

Abstract: This work evaluates the activity of a few key enzymes involved in combating reactive oxygen species (ROS), such as ascorbate peroxidase (EC 1.11.1.11), catalase (EC 1.11.1.6), glutathione reductase (EC 1.6.4.2), and superoxide dismutase (EC 1.15.1.1), as well as the concentration of malondialdehyde and hydrogen peroxide in transgenic and non-transgenic soybean leaves. Additionally, differential protein species …

Abstract: Paired-choice assays in a tritrophic system have been carried out to study the influence of transgenic Bacillus thuringiensisvar. kurstaki (Berliner) corn plants on prey preference of the predator Chrysoperla carnea (Stephens). Plants used were a transgenic B. thuringiensis-expressing (Cry1Ab) corn hybrid and the corresponding isogenic untransformed B. thuringiensis-free hybrid. Two different prey species were …

Abstract: Improvements in the performance of fish species used in aquaculture are being accomplished using a variety of approaches, including both traditional and molecular genetic methodologies. Historical gains in productivity have been achieved by domestication, selection, interspecific and interstrain crossbreeding, polyploidy, and synthesis of monosex populations. More recently, transgenesis has been explored as a technique …

Abstract: To ascertain whether transgenic engineering will affect resistance of seeds and seedlings to stresses, and consequently provide first-hand scientific information for environmental safety assessment of transgenic soybean, comparative studies were conducted in laboratory and greenhouse on transgenic soybean ARG, it′s counterpart n-ARG and Su 3 a traditional soybean cultivar in China. Results show at …

Abstract: Many researchers have hailed transgenic insecticidal crops—plants modified to produce insecticidal proteins derived from genes of the bacterium Bacillus thuringiensis (Bt)—as the most important technological advancement in insect pest management since the development of synthetic insecticides (Vaeck et al. 1987, Koziel et al. 1993, Perlak et al. 1990, 1993). At least 18 transgenic insecticidal …

Abstract: The potential impact of transgenic crops on community ecology will depend on the distribution and establishment of the new transgenic traits, on the sexual transfer of their new genes to the environment (Bartsch & Pohl-Orf, 1996) and on the potential ecological impact of the transgenic trait. Flowering and pollen dispersal is important for outcrossing …

Conclusions: 1. The current high-dose strategy will reduce densities of natural enemies that tend to be more host specific, perhaps to a greater extent than the use of insecticides. The elimination of target species may cause local extinctions of host-specific natural enemies, creating metapopulation dynamics. As discussed previously (Bernal et al., 2002), the widespread use …

Abstract: Background Transgenic proteins expressed by genetically modified food crops are evaluated for their potential allergenic properties prior to marketing, among others by identification of short identical amino acid sequences that occur both in the transgenic protein and allergenic proteins. A strategy is proposed, in which the positive outcomes of the sequence comparison with a …

Abstract: A tracer experiment is carried out with transgenic T (variety M 7211 RR) and non-transgenic NT (variety MSOY 8200) soybean plants to evaluate if genetic modification can influence the uptake and translocation of Fe. A chelate of EDTA with enriched stable 57Fe is applied to the plants cultivated in vermiculite plus substrate and the …

Abstract: The transgenic and non-transgenic cotton seed oil were used as materials to analyze the composition and concentration of fatty acids. The results showed that 16 kinds of fatty acids were detected in transgenic cotton seed oil, and 15 kinds of fatty acids in non-transgenic cotton seed oil; concentration of most of the 15 kinds …

Abstract: Integration of transgenic DNA into the plant genome was investigated in 13 transgenic oat (Avena sativa L.) lines produced using microprojectile bombardment with one or two cotransformed plasmids. In all transformation events, the transgenic DNA integrated into the plant genome consisted of intact transgene copies that were accompanied by multiple, rearranged, and/or truncated transgene …

Abstract: Soil properties, microbial communities, and enzyme activities were studied in soil planted with transgenic or nontransgenic papaya under field conditions. The transgenic papaya contained a replicase (RP) mutant gene of the papaya ringspot virus (PRSV), which conferred resistance to the virus, the neomycin phosphotransferase II (NPT II) marker gene, which conferred Km resistance, and …

Abstract: The neo (neomycin phosphotransferase) gene is widely used as a selection marker in the production of genetically engineered animals and plants. Recent attention has been focused on safety concerns regarding neo transgene expression. In this study, neo transgenic and non-transgenic piglets were randomly assigned into Group A and Group B to evaluate effects of …

Abstract: The transgenic and non-transgenic soybean seeds oils were extracted and the composition and concentration of the fatty acids were analyzed by gas chromatography. Sixteen different fatty acids were detected in both transgenic and non-transgenic soybean oil: C14:0, C16:0, C18:1, C18:2, C18:3, C20:0, C20:1, C20:2, C22:0, C20:5, C22:1, C24:0 and C22:6. The concentration of 12 of …

Abstract: Genetic engineering, also called genetic modification, is facing with growing demands of aquaculture and aquatic products. Although various genetically modified (GM) aquatics have been generated, it is important to evaluate biosafety of GM organisms on the human health before entering into our food chain. For this purpose, we establish a zebrafish wild adult feeding …

Abstract: Genetically modified plants are widely grown predominantly in North America and to a lesser extent in Australia, Argentina and China but their regions of production are expected to spread soon beyond these limited areas also reaching Europe where great controversy over the application of gene technology in agriculture persists. Currently, several cultivars of eight …

Abstract: Bacillus thuringiensis (Bt) transgenic cotton is the unique Bt transgenic crop planted on a large scale in China, and its commercialized varieties and hectareage had increased rapidly in China during the past decade (1997–2006) with broad geographic distribution for the economic, environmental, and health benefits. In 2004, the planting area of Bt transgenic cotton in China …