Phau ntawv xov xwm lossis Publishing Institution: Theoretical thiab Applied Genetics
Kawm: http://link.springer.com/article/10.1007%2Fs00122-003-1278-0
Tus sau: Warwick, SI, Simard, MJ, Légère, A., Beckie, HJ, Braun, L., Zhu, B., Mason, P., Séguin-Swartz, G. and Stewart, CN
Tshooj Lus: Peer tshuaj xyuas
Daim ID: 1041
Abstract: Qhov zaus ntawm cov noob caj noob ces los ntawm Brassica nplooj L. (canola) rau plaub tus txheeb ze qus, Brassica paj L., Tooj Cua Raphanistrum L., Sinapis arvensis L. thiab Erucastrum gallicum (Willd.) OE Schulz, tau raug soj ntsuam hauv kev sim tsev cog khoom thiab/lossis thaj teb, thiab cov nqi tiag tiag ntsuas hauv cov teb lag luam hauv Canada. Ntau lub cim qhia tau siv los ntes cov tib neeg sib xyaw: cov yam ntxwv tiv thaiv tshuaj tua kab (HR), cov cim protein fluorescent ntsuab (GFP), cov qauv tshwj xeeb ntawm cov hom kab amplified fragment length polymorphisms (AFLPs) thiab ploidy level. Kev sib xyaw ntawm B. rov thiab B. noj tau tshwm sim hauv ob qhov kev sim hauv thaj teb (zaus kwv yees li 7%) thiab hauv cov pej xeem qus hauv cov teb lag luam (kwv yees li 13.6%). Qhov ntau dua hauv cov teb lag luam feem ntau yog vim muaj qhov deb ntawm B. rov cov nroj tsuag. Tag nrho F1 cov hybrids tau zoo sib xws morphologically rau B. rov, muaj B. noj- thiab B. rov-cov cim AFLP tshwj xeeb thiab yog triploid (AAC, 2n = 29 chromosomes). Lawv tau txo qis kev muaj peev xwm ntawm paj ntoos (kwv yees li 55%) thiab cais rau ob qho tib si tus kheej tsis sib xws thiab tus kheej sib xws (qhov kawg yog ib qho B. noj tus cwj pwm). Qhov sib txawv, cov noob caj noob ces ntawm R. raphanistrum thiab B. noj tsis tshua muaj. Ib leeg xwb R. raphanistrum × B. noj F1 tau pom cov noob sib xyaw ua ke hauv 32,821 cov noob cog los ntawm HR B. noj kev sim ua teb. Cov hybrid zoo ib yam li R. raphanistrum tsuas yog muaj cov valve xwb, ib qho B. noj tus cwj pwm, nyob rau hauv cov noob pods distorted. Nws muaj ib tug genomic qauv sib xws nrog lub fusion ntawm ib tug unreducated gamete ntawm R. raphanistrum thiab ib qho gamete txo qis ntawm B. noj (RrRrAC, 2n = 37), ob qho tib si B. noj- thiab R. raphanistrum-cov cim AFLP tshwj xeeb, thiab muaj <1% paj ntoos muaj sia nyob. Tsis muaj cov hybrids tau pom nyob rau hauv cov kev sim tsev cog khoom (1,534 cov noob), GFP teb sim (4,059 cov noob) lossis hauv cov teb lag luam hauv Québec thiab Alberta (22,114 cov noob). Tsis muaj S. arvensis or E. gallicum × B. noj Cov noob sib xyaw tau pom (42,828 thiab 21,841 noob, feem) los ntawm cov teb lag luam hauv Saskatchewan. Cov kev tshawb pom no qhia tias qhov muaj feem yuav muaj cov noob hloov pauv los ntawm cov noob hloov pauv B. noj rau R. raphanistrum, S. arvensis or E. gallicum qis heev (<2–5 × 10-5). Txawm li cas los xij, cov transgenes tuaj yeem tawg mus rau hauv ib puag ncig los ntawm kev qus B. rov nyob rau sab hnub tuaj Canada thiab tej zaum los ntawm kev lag luam B. rov cov neeg ua haujlwm pab dawb hauv sab hnub poob Canada.
keywords: Cov canola transgenic, Rapeseed, Kev ua phem rau noog, Mustard dev, Zaub ntsuab qus, Mustard qus, Gene flow, Cov teb ua lag luam loj
Citation: Warwick, SI, Simard, MJ, Légère, A., Beckie, HJ, Braun, L., Zhu, B., Mason, P., Séguin-Swartz, G. and Stewart, CN, 2003. Hybridization between transgenic Brassica napus L. and its wildrapa. L. Brassica. Sinapis arvensis L., thiab Erucastrum gallicum (Willd.) OE Schulz. Theoretical thiab Applied Genetics, 107(3), phab 528-539.
