Žurnalas ar leidybos institucija: Vandens toksikologija
Tyrimas: https://www.sciencedirect.com/science/article/pii/S0166445X17301315
Autorius (-iai): Li, M.H., Ruan, L.Y., Zhou, J.W., Fu, Y.H., Jiang, L., Zhao, H. and Wang, J.S.
Straipsnio tipas: Kolegų peržiūrėtas tyrimas
Įrašo ID: 1414
Abstract: Glyphosate is an efficient herbicide widely used worldwide. However, its toxicity to non-targeted organisms has not been fully elucidated. In this study, the toxicity of glyphosate-based herbicide was evaluated on goldfish (Carassius auratus) after long-term exposure. Tissues of brains, kidneys and livers were collected and submitted to NMR-based metabolomics analysis and histopathological inspection. Plasma was collected and the blood biochemical indexes of AST, ALT, BUN, CRE, LDH, SOD, GSH-Px, GR and MDA were measured. Long-term glyphosate exposure caused disorders of blood biochemical indexes and renal tissue injury in goldfish. Metabolomics analysis combined with correlation network analysis uncovered significant perturbations in oxidative stress, energy metabolism, amino acids metabolism and nucleosides metabolism in glyphosate dosed fish, which provide new clues to the toxicity of glyphosate. This integrated metabolomics approach showed its applicability in discovering the toxic mechanisms of pesticides, which provided new strategy for the assessment of the environmental risk of herbicides to non-target organisms.
Keywords: Glyphosate, Toxicity, NMR-Based Metabolomics, OSC-PLS-DA, Correlation Network; Carassius Auratus, Alanine Transaminase, Aspartate Transaminase, Blood, Chronic Exposure, Energy Metabolism, Gluathione Peroxidase, Goldfish, Histopathology, Kidneys, Liver, Metabolomics, Nontarget Organisms, Nucleosides, Oxidative Stress, Risk, Superoxide Dismutase, Tissues; Animals, Brain, Energy Metabolism, Glycine, Herbicides, Magnetic Resonance Spectroscopy, Male, Chemical Water Pollutants; Metabolism, Drug Effects
Citation: Li, M.H., Ruan, L.Y., Zhou, J.W., Fu, Y.H., Jiang, L., Zhao, H. and Wang, J.S., 2017. Metabolic profiling of goldfish (Carassius auratis) after long-term glyphosate-based herbicide exposure. Vandens toksikologija, 188, pp.159-169.