GMO Research
Abstract: In his clinical review on genetically modified foods Jones implies that Pusztai had tested only the effects of potato spiked with concanavalin A (a lectin) at the Rowett Institute. The initial dissemination of this incorrect information followed by the inappropriate suspension of Pusztai and the suspicion of fraud implied by instituting an audit according …
Abstract: The inclusion of genetically modified (GM) plants in the human diet has raised concerns about the possible transfer of transgenes from GM plants to intestinal microflora and enterocytes. The persistence in the human gut of DNA from dietary GM plants is unknown. Here we study the survival of the transgene epsps from GM soya …
Abstract: Growth hormone (GH) gene expression has been examined in control and transgenic coho salmon containing a transgene comprised of the sockeye salmon GH1 gene under the control of the MT-B promoter from the same species. This transgene dramatically enhances the growth of salmonids, and raises serum GH levels some forty-fold. Transcript levels from this …
Abstract: Research purpose. Examine under conditions in vitro effect of aqueous extract of GM roundup resistant soybean compared with soy classical breeding activity propionic acid bacteria are used in starters in the manufacture of hard cheeses with a long shelf life, and the use of transgenic soybeans in feeding chicks and laying hens with the …
Abstract: Genetic engineering, also called genetic modification, is facing with growing demands of aquaculture and aquatic products. Although various genetically modified (GM) aquatics have been generated, it is important to evaluate biosafety of GM organisms on the human health before entering into our food chain. For this purpose, we establish a zebrafish wild adult feeding …
Abstract: The neo (neomycin phosphotransferase) gene is widely used as a selection marker in the production of genetically engineered animals and plants. Recent attention has been focused on safety concerns regarding neo transgene expression. In this study, neo transgenic and non-transgenic piglets were randomly assigned into Group A and Group B to evaluate effects of …
Abstract: The objective of this study was to evaluate whether standard fish meal diets prepared with increasing levels of genetically modified (GM; 150 and 300 g kg−1) maize (event MON810®) as a starch source, showed any nutritional or physiological adverse effects on Atlantic salmon, Salmo salar L. postsmolt. The diets with low or high inclusions of GM …
Abstract: Glyphosate (N-(phosphonomethyl) glycine) is a most popular herbicide in agricultural practices throughout the world. It is possible that glyphosate spread in the ecosystems can reach plants, animals. The present work was directed to investigate the glyphosate residue in different organs of broiler chickens using ELISA and to study the possibility of its neutralisation using …
Abstract: The present experiment was conducted to study the possible effects of genetically modified (GM), full‐fat soybean meal (FFSBM) from Round‐up Ready® soybeans compared to its parental, and closest near‐isogenic, non‐modified (nGM) soybean variety, added at moderate (150 g kg−1) and high (300 g kg−1) inclusion levels. The fish showed a high specific growth rate (SGR …
Abstract: The 90-day animal study is the core study for the safety assessment of genetically modified foods in the SAFOTEST project. The model compound tested in the 90-day study was a rice variety expressing the kidney bean Phaseolus vulgaris lectin agglutinin E-form (PHA-E lectin). Female Wistar rats were given a nutritionally balanced purified diet with …
Abstract: Genetically modified (GM) crops have been commercially available for human and animal consumption since the 1990s. The safety evaluations are based on the concept of substantial equivalence, which assumes that the toxicity of a product can be investigated by assessing the toxicity of individual components of the product and not the product as a …
Abstract: The article is devoted to the introduction of transgenic products into the consumer market. The impact of the genetically modified potatoes on the cytological and morphological parameters of mice was investigated. Transgenic potato plants were produced by agrobacterium-mediated transformation using genetic constructions 35S-proSmAMP with marker gene npt11 of the resistance to the kanamycin. An increase in hearts …
Abstract: In the present study glyphosate residues were tested in urine and different organs of dairy cows as well as in urine of hares, rabbits and humans using ELISA and Gas Chromatography-Mass Spectroscopy (GC-MS). The correlation coefficients between ELISA and GC-MS were 0.96, 0.87, 0.97 and 0.96 for cattle, human, and rabbit urine and organs, …
Abstract: As part of the SAFOTEST project the immunmodulating effect of Cry1Ab protein from Bacillus thuringiensis (Bt) and PHA-E lectin from kidney bean (Phaseolus vulgaris erythroagglutinin) was examined in 28- and 90-day feeding studies in Wistar rats. PHA-E lectin was chosen as positive control. Rats were fed control rice, transgenic rice expressing Cry1Ab protein or …
Abstract: The effects of phenoxyacid herbicides 2,4‐D (2,4‐dichlorophenoxyacetic acid) and MCPA (4‐chloro‐2‐methylphenoxyacetic acid), clofibrate, and glyphosate on hepatic and intestinal drug metabolizing enzyme activities were studied in rats intragastrically exposed for 2 weeks. The hepatic ethoxycoumarin O‐deethylase activity increased about 2‐fold with MCPA. Both 2,4‐D and MCPA increased the hepatic epoxide hydrolase activity and decreased …
Abstract: The present paper represents a part of a major scientific effort aiming to reveal possible effects, nutritional or health related, of genetically modified (GM) feed ingredients for Atlantic salmon. For 3 months groups of post‐smolt Atlantic salmon were treated with diets holding 130 g kg1 of the protein as soybean, one which contained 800 g kg−1 GM type RR …
Abstract: Responses to GM maize Bt-maize, MON810) expressing Cry1Ab protein from the soil bacterium Bacillus thuringiensis (Bt) in diets for both normal and immune-sensitised (with soyabean meal (SBM)-induced enteropathy) post-smolt Atlantic salmon were investigated following 33 and 97 d of exposure. Triplicate tanks of salmon were fed one of four diets, all containing 20% whole-kernel …
Abstract: The presence of antibiotic resistance marker genes in genetically engineered plants is one of the most controversial issues related to Genetically Modified Organism (GMO)-containing food, raising concern about the possibility that these markers could increase the pool of antibiotic resistance genes. This study investigates the in vitro survival of genes bla and cryIA(b) of …
Abstract: A new concept for viral oncogenesis is presented which is based on experimental work on the chromosomal integration of adenovirus DNA into mammalian genomes. The mechanism of adenovirus DNA integration is akin to non-sequence-specific insertional recombination in which patch homologies between the recombination partners are frequently observed. This reaction has been imitated in a …
Abstract: Tilapias (Oreochromis niloticus) were fed using transgenic soybean diets and non-transgenic soybean diets. Gastric contents, intestinal contents and faeces were sampled respectively from tilapias at 1h, 4h and 8h after feeding and different tissues of tilapias were collected at 4-, 7-week and continued hunger 2-week. DNA was extracted from these samples and the distribution …
Abstract: During 17 years when Bt-crops were used on an industrial scale, eight species of pests (seven species of lepidopterans from the order Lepidoptera belonging to four families and one species of coleopterous insects from the order Coleoptera) developed resistance to them. In five cases, the mutation of resistance was so widespread that it caused …
Abstract: Insecticidal crystal toxins derived from the soil bacterium Bacillus thuringiensis (Bt) are widely used as biopesticide sprays or expressed in transgenic crops to control insect pests. However, large-scale use of Bt has led to field-evolved resistance in several lepidopteran pests. Resistance to Bt Cry1Ac toxin in the diamondback moth, Plutella xylostella (L.), was previously …
