Journal or Publishing Institution: International Journal of Current Microbiology and Applied Sciences
Author(s): Stefanova, P., Angelova, G., Georgieva, T., Gotcheva, V. and Angelov, A.
Article Type: Peer Reviewed Study
Record ID: 2209
Abstract: The increasing number of commercialized GM crops and the growing need for authenticity control of raw materials, feeds and foods set an urgent necessity for the development of sensitive, reliable and cost-effective methods for GMO detection. In the present study, a novel multiplex PCR method for the simultaneous detection of all EU-authorized genetically modified soybean events was developed. The method was based on three gene-specific (EPSPS, PAT and Cry1Ac genes) and one event-specific (DP 356043) DNA sequences. It was characterized with high sensitivity, as the LOD for each sequence was 0.05%. The new method was applied for the screening of 15 soybean products and 36 meat products at the market for the presence of genetically modified soybean events. Results demonstrated that 51% of the tested samples contained EPSPS gene, while PAT gene was detected in 8% of the DNA extracts. In contrast to that, Cry1Ac gene and DP 356043 event-specific sequence were not observed in any of the analyzed products. The data indicated that the proposed method could be used as a reliable routine screening assay of various food products for the presence of EU-authorized genetically modified soybean events.
Keywords: Multiplex PCR, Genetically modified soybean events, EU legislation, Soybean products, Meat products
Citation: Stefanova, P., Angelova, G., Georgieva, T., Gotcheva, V. and Angelov, A., 2015. A novel multiplex PCR method for simultaneous detection of genetically modified soybean events. International Journal of Current Microbiology and Applied Sciences, 4(4), pp.256-268.
