Antibiotic resistance marker genes as environmental pollutants in GMO-pristine agricultural soils in Austria

Journal or Publishing Institution: Environmental Pollution

Study: https://www.sciencedirect.com/science/article/pii/S0269749115003619?via%3Dihub

Author(s): Woegerbauer, M., Zeinzinger, J., Gottsberger, R.A., Pascher, K., Hufnagl, P., Indra, A., Fuchs, R., Hofrichter, J., Kopacka, I., Korschineck, I. and Schleicher, C.

Article Type: Peer Reviewed Study

Record ID: 1255

Abstract: Antibiotic resistance genes may be considered as environmental pollutants if anthropogenic emission and manipulations increase their prevalence above usually occurring background levels. The prevalence of aph(3′)-IIa/nptII and aph(3′)-IIIa/nptIII – frequent marker genes in plant biotechnology conferring resistance to certain aminoglycosides – was determined in Austrian soils from 100 maize and potato fields not yet exposed to but eligible for GMO crop cultivation. Total soil DNA extracts were analysed by nptII/nptIII-specific TaqMan real time PCR. Of all fields 6% were positive for nptII (median: 150 copies/g soil; range: 31–856) and 85% for nptIII (1190 copies/g soil; 13–61600). The copy-number deduced prevalence of nptIII carriers was 14-fold higher compared to nptII. Of the cultivable kanamycin-resistant soil bacteria 1.8% (95% confidence interval: 0–3.3%) were positive for nptIII, none for nptII (0–0.8%). The nptII-load of the studied soils was low rendering nptII a typical candidate as environmental pollutant upon anthropogenic release into these ecosystems.

Keywords: Resistance, Aminoglycosides, Antibiotics, Soil contamination, Real time PCR

Citation: Woegerbauer, M., Zeinzinger, J., Gottsberger, R.A., Pascher, K., Hufnagl, P., Indra, A., Fuchs, R., Hofrichter, J., Kopacka, I., Korschineck, I. and Schleicher, C., 2015. Antibiotic resistance marker genes as environmental pollutants in GMO-pristine agricultural soils in Austria. Environmental Pollution, 206, pp.342-351.