Journal or Publishing Institution: Trends in Genetics
Study: https://www.sciencedirect.com/science/article/pii/S016895252030247X
Author(s): Burgio, G. and Teboul, L.
Article Type: Journal Publication
Abstract:
Genome editing has powerful applications in research, healthcare, and agriculture. However, the range of possible molecular events resulting from genome editing has been underestimated and the technology remains unpredictable on, and away from, the target locus. This has considerable impact in providing a safe approach for therapeutic genome editing, agriculture, and other applications. This opinion article discusses how to anticipate and detect those editing events by a combination of assays to capture all possible genomic changes. It also discusses strategies for preventing unwanted effects, critical to appraise the benefit or risk associated with the use of the technology. Anticipating and verifying the result of genome editing are essential for the success for all applications.
Keywords: genome editing, on-target activity, off-target activity, ectopic insertions, validation
Citation:
Burgio, G. and Teboul, L., 2020. Anticipating and identifying collateral damage in genome editing. Trends in Genetics, 36(12), pp.905-914.
