Journal or Publishing Institution: PAU Ludhiana
Study: https://web.archive.org/web/20180801182612/http://krishikosh.egranth.ac.in/handle/1/5810025605
Author(s): Kansal, M.
Article Type: Study
Record ID: 1200
Abstract: The present study was designed to evaluate the impact of feeding a line of genetically modified cotton seeds (Bt cotton seeds) to that of standard diet and reference diet (Non-Bt cotton seeds) on growth, metabolism in albino rats. Non-Bt and Bt cotton seeds were incorporated into the diet of the albino rats at a concentration of 20% continuously for three generations. The results of the multigenerational feeding studies included reproductive performance, growth rate, litter size, organ weights, histological, biochemical and electron microscopic ultrastructural investigations to detect changes at the organ and cellular level between control, Non-Bt and Bt cotton seed fed animals. The production parameters, average litter size as well as number of weaned pups were in favour of the control and Non-Bt fed lines. Relative weights of liver, heart, kidney were observed to be higher in Bt fed female rats in all the three generations as compared to control and Non-Bt group. Light microscopic observation of the liver and kidney sections of cottons seeds fed female and male rats showed many histopathological changes. The electron microscopic examination of liver revealed the presence of the sinusoidal endothelium with large endothelial „gaps‟ in Bt fed rats. Levels of total proteins, albumin, globulin, uric acid, creatinine was found to be higher in plasma of Bt group female and male rats of all the three generations. The values of liver function enzymes i.e. aminotransferases was non-significantly higher in Bt fed group rats as compared with the enzyme values observed in the Non-Bt group. A statistically significant increase in total proteins, total lipids and cholesterol in ovaries of Bt group rats were observed as compared to control rats.
Keywords: Bt cotton seeds, albino rat, liver, kidney, ovary, testis, enzymes, proteins, lipids, reproductive performance, rats, rodent, rodents, GMO, GM, genetically modified, genetically engineered, feeding, transgenic
Citation: Kansal, M., 2013. Biological effects of transgenic Bt cotton seeds fed in long term reproduction studies in rat. Doctoral Dissertation, PAU Ludhiana.
