Journal or Publishing Institution: Trends in Plant Science
Date of Publication: 01/01/2017
Study: https://www.ncbi.nlm.nih.gov/pubmed/27645899
Author(s): Zhu, C., Bortesi, L., Baysal, C., Twyman, R.M., Fischer, R., Capell, T., Schillberg, S. and Christou, P.
Article Type: Peer Reviewed Study
Abstract:
Designer nucleases allow the creation of new plant genotypes by introducing precisely-targeted double-strand breaks that are resolved by endogenous repair pathways. The major nuclease technologies are meganucleases, zinc-finger nucleases, transcription activator-like effector nucleases, and the CRISPR/Cas9 system. Each comprises a promiscuous endonuclease guided by protein-DNA or RNA-DNA interactions. A great deal is known about the principles of designer nucleases but much remains to be learned about their detailed behavioral characteristics in different plant species. The outcome of genome engineering reflects the intrinsic properties of each nuclease and target genome, causing variations in efficiency, accuracy, and mutation structure. In this article, we critically discuss the activities of designer nucleases in different cereals representing a broad range of genome characteristics.
Keywords: gene editing, plant breeding, genomic damage, CRISPR/Cas9, designer nucleases
Citation:
Zhu, C., Bortesi, L., Baysal, C., Twyman, R.M., Fischer, R., Capell, T., Schillberg, S. and Christou, P., 2017. Characteristics of genome editing mutations in cereal crops. Trends in Plant Science, 22(1), pp.38-52.
Category:
- Health effects
Record ID: 2524