Cytogenetic damage and induction of pro-oxidant state in human lymphocytes exposed in vitro to gliphosate, vinclozolin, atrazine, and DPX-E9636

Journal or Publishing Institution: Environmental and Molecular Mutagenesis

Study: http://www.ncbi.nlm.nih.gov/pubmed/9707097

Author(s): Lioi, M.B., Scarfi, M.R., Santoro, A., Barbieri, R., Zeni, O., Salvemini, F., Di Berardino, D. and Ursini, M.V.

Article Type: Peer Reviewed Study

Record ID: 1425

Abstract: We analyzed chromosome aberrations (CAs), sister chromatid exchanges (SCEs), mitotic index (MI), and glucose 6-phosphate dehydrogenase (G6PD) enzyme activity in human peripheral lymphocytes from three healthy donors exposed in vitro to different concentrations of gliphosate, vinclozolin, atrazine, and DPX-E9636. The pesticides gliphosate, vinclozolin, and atrazine have been studied in a broad range of genetic tests with predominantly conflicting or negative results, whereas little is known about the genotoxicity of DPX-E9636. In our experimental conditions, each chemical compound tested produced a dose-related increase in the percent of aberrant cells and an increase of SCE/cell. Furthermore, at the highest concentrations of vinclozolin, atrazine, and DPX-E9636, we observed a significant reduction of the mitotic index. The increase of G6PD activity in exposed lymphocyte cultures strongly indicated an induction of a pro-oxidant state of the cells as an initial response to pesticide exposure.

Keywords: Atrazine, Cultured Cells, Chromosome Aberrations, Glucosephosphate Dehydrogenase, Humans, Lymphocytes, Mutagens, Oxazoles, Oxidants, Oxidative Stress, Pesticides, Sister Chromatid Exchange, Urea, Vinclozolin; Toxicity, Metabolism, Drug Effects, Enzymology; Genotoxicity, Sister Chromatid Exchanges, Glucose 6-Phosphate Dehydrogenase

Citation: Lioi, M.B., Scarfi, M.R., Santoro, A., Barbieri, R., Zeni, O., Salvemini, F., Di Berardino, D. and Ursini, M.V., 1998. Cytogenetic damage and induction of pro‐oxidant state in human lymphocytes exposed in vitro to gliphosate, vinclozolin, atrazine, and DPX‐E9636. Environmental and Molecular Mutagenesis, 32(1), pp.39-46.