Journal or Publishing Institution: International Journal of Hygiene and Environmental Health
Study: http://www.ncbi.nlm.nih.gov/pubmed/16373198
Author(s): Šiviková, K. and Dianovský, J.
Article Type: Peer Reviewed Study
Record ID: 2135
Abstract: A technical herbicide containing isopropyl amine salt of glyphosate was tested for induction of chromosome aberrations (CA) and sister chromatid exchanges (SCE) in cultured bovine peripheral lymphocytes. Cultures were exposed to a glyphosate formulation at concentrations ranging from 28 to 1120 micromol/l without and with metabolic activation. No clastogenic effect of the herbicide was found. Its genotoxic effect was confirmed in the SCE assay after 24 h of incubation. A statistically significant elevation in SCE induction was observed in each of the donors after application of the product at doses ranging from 56 to 1120 micromol/l. The highest concentrations (560 and 1120 micromol/l) also caused reduction of mitotic and proliferation indices. In the 2 h-assay with metabolic activation a statistically significant frequency of SCE was observed only in cultures treated with the agent at a concentration of 140 micromol/l.
Keywords: Bovine peripheral lymphocytes, Sister chromatid exchanges, Glyphosate, Chromosome aberration, Animals, Cattle, Cell Proliferation, Cultured Cells, Chromosome Aberrations, Mammalian Chromosomes, Glycine, Lymphocytes, Mitotic Index, Sister Chromatid Exchange, Drug Effects, Analogs & Derivatives, Toxicity
Citation: Šiviková, K. and Dianovský, J., 2006. Cytogenetic effect of technical glyphosate on cultivated bovine peripheral lymphocytes. International Journal of Hygiene and Environmental Health, 209(1), pp.15-20.
