Journal or Publishing Institution: Transgenic Research
Study: http://link.springer.com/article/10.1007/s11248-009-9339-z#page-1
Author(s): Paul, V., Guertler, P., Wiedemann, S. and Meyer, H.H.
Article Type: Peer Reviewed Study
Record ID: 1839
Abstract: To investigate the relative degradation and fragmentation pattern of the recombinant Cry1Ab protein from genetically modified (GM) maize MON810 throughout the gastrointestinal tract (GIT) of dairy cows, a 25 months GM maize feeding study was conducted on 36 lactating Bavarian Fleckvieh cows allocated into two groups (18 cows per group) fed diets containing either GM maize MON810 or nearly isogenic non-GM maize as the respective diet components. All cows were fed a partial total mixed ration (pTMR). During the feeding trial, 8 feed (4 transgenic (T) and 4 non-transgenic (NT) pTMR) and 42 feces (26 T and 18 NT) samples from the subset of cows fed T and NT diets, and at the end of the feeding trial, digesta contents of rumen, abomasum, small intestine, large intestine and cecum were collected after the slaughter of six cows of each feeding group. Samples were analyzed for Cry1Ab protein and total protein using Cry1Ab specific ELISA and bicinchoninic acid assay, respectively. Immunoblot analyses were performed to evaluate the integrity of Cry1Ab protein in feed, digesta and feces samples. A decrease to 44% in Cry1Ab protein concentration from T pTMR to the voided feces (9.40 versus 4.18 μg/g of total proteins) was recorded. Concentrations of Cry1Ab protein in GIT digesta of cows fed T diets varied between the lowest 0.38 μg/g of total proteins in abomasum to the highest 3.84 μg/g of total proteins in rumen. Immunoblot analysis revealed the extensive degradation of recombinant Cry1Ab protein into a smaller fragment of around 34 kDa in GIT. The results of the present study indicate that the recombinant Cry1Ab protein from MON810 is increasingly degraded into a small fragment during dairy cow digestion.
Keywords: Cry1Ab Protein, Dairy Cow, Genetically Modified Maize MON810, Digesta; Cattle, Plants, Zea mays, Cattle Feeding, Cows, Dairy Cattle, Dairy Cows, Diets, Digesta, Digestion, Digestive Tract, Feeds, Genetically Engineered Organisms, Lactation, Maize, Protein, Protein Degradation, Protein Digestibility, Protein Digestion, Recombinant Proteins, Transgenic Plants; Corn, Digestive Tract Contents, Feeding Stuffs, Gastrointestinal Tract, Genetically Engineered Plants, Genetically Modified Organisms, Genetically Modified Plants, GEOs, GMOs, Transgenic Organisms; Simmental abomasum, Cecum, Crystal Proteins, Enzyme-Linked Immunosorben Assay, Feces, Insecticidal Proteins, Lactation, Protein Content, Rumen, Slaughter, Small Intestine, Total Mixed Rations; Animal Feed, Animals, Bacterial Proteins, Cattle, Dairying, Dietary Proteins, Endotoxins, Genetically Modified Food, Hemolysin Proteins, Post-Translation Protein Processing
Citation: Paul, V., Guertler, P., Wiedemann, S. and Meyer, H.H., 2010. Degradation of Cry1Ab protein from genetically modified maize (MON810) in relation to total dietary feed proteins in dairy cow digestion. Transgenic Research, 19(4), pp.683-689.
