Journal or Publishing Institution: Mutagenesis
Date of Publication: 07/01/2007
Study: http://mutage.oxfordjournals.org/content/22/4/263.full
Author(s): Cavas, T., and Konen, S.
Article Type: Peer Reviewed Study
Abstract:
Glyphosate is a widely used broad-spectrum weed control agent. In the present study, an in vivo study on the genotoxic effects of a technical herbicide (Roundup®) containing isopropylamine salt of glyphosate was carried out on freshwater goldfish Carassius auratus. The fish were exposed to three doses of glyphosate formulation (5, 10 and 15 ppm). Cyclophosphamide at a single dose of 5 mg/l was used as positive control. Analysis of micronuclei, nuclear abnormalities and DNA damage were performed on peripheral erythrocytes sampled at intervals of 48, 96 and 144 h posttreatment. Our results revealed significant dose-dependent increases in the frequencies of micronuclei, nuclear abnormalities as well as DNA strand breaks. Our findings also confirmed that the alkaline comet assay and nuclear deformations in addition to micronucleus test on fish erythrocytes in vivo are useful tools in determining the potential genotoxicity of commercial herbicides.
Keywords: cyclophosphamide, erythrocytes, comet assay, cytogenetics, DNA, genotoxicity, goldfish, herbicides, pesticides, micronucleus, micronucleus tests, glyphosate, positive control, fish
Citation:
Cavas, T., and Konen, S., 2007. Detection of cytogenetic and DNA damage in peripheral erythrocytes of goldfish (Carassius auratus) exposed to a glyphosate formulation using the micronucleus test and the comet assay. Mutagenesis, 22(4):263- 268.
Category:
- Environmental effects
- Pesticide use
Record ID: 318
