Detection of genetically modified DNA in milk using species specific PCR

Journal or Publishing Institution: Journal of American Science

Study: https://web.archive.org/web/20180728182956/http://www.jofamericanscience.org/journals/am-sci/am0809/036_10402am0809_248_252.pdf

Author(s): Hassan, K.I. and Ali, A.A.

Article Type: Peer Reviewed Study

Record ID: 936

Abstract: Milk has many nutrients that make it an ideal food all over the world and Iraq is one of the developing countries who imports milk to supply the people with this important food.And because most milk producer in the world depend on genetically modified (GM) feed to fed their animals, so these GM DNA may transfer to the products of these animals including milk. To detect of the purity of milk from GM DNA fragments this study was carried out,by collecting seven Samples of imported milk powder from the Sulaimani market in Iraq, which include: Anchor, Mudhish, Maraey Al-khadra, Dielac (Ireland), |Nido, Premier and Dielac (Neusland) and a fresh milk sample from cow, sheep and goat from private farm k .Suitable amount of DNA was obtained using by CTAB method from all the samples, To evaluate the feasibility of the PCR method for detection of GM DNA in milk, Species specific primer targeting the most common GM gene associated with feed including NOS-terminator of bar inserted gene in herbicide tolerant Event 176 GM-maize and the 35S-promoter of EPSPS inserted gene in pest resistance Roundup Ready GM-soybeans. The presence of the transgenic sequences of maize of the expected size (184bp) was reproducibly amplified in three samples whereas no amplified band was obtained using the other set of primers which are specific to the GM-soybeans. The data revealed that the PCR method can sufficiently detect GM DNA in milk powder to differentiate the GM Products from non-GM one.

Keywords: GM DNA, Detection, Milk powder, PCR, species specific, transgenic sequences of maize

Citation: Hassan, K.I. and Ali, A.A., 2012. Detection of genetically modified DNA in milk using species specific PCR. Journal of American Science, 8(9), pp.248-252.