Journal or Publishing Institution: Veterinary and Human Toxicology
Study: https://www.ncbi.nlm.nih.gov/pubmed/12678298
Author(s): Chowdhury, E.H., Mikami, O., Nakajima, Y., Hino, A., Kuribara, H., Suga, K., Hanazumi, M. and Yomemochi, C.
Article Type: Peer Reviewed Study
Abstract:
We tried to detect DNA fragments derived from maize in the intestinal contents of pigs fed genetically modified (GM) StarLink CBH351 maize (SL) or non-GM maize. Intestinal contents of 8 SL and 8 non-GM maize-fed pigs were collected at slaughter, and the genes of the recombinant cry9C and the maize intrinsic zein (Zel) were assayed by polymerase chain reaction (PCR) 3 times with a total of 4 primer pairs of different expected lengths. The cry9C gene (either 103 or 170 bp) was detected in the rectal contents (with a frequency of 25-37.5%) and in the cecal contents (25-50%) of the pigs fed SL. In a similar fashion, the zein (Zel) gene (either 242 or 329 bp) was detected in the rectal contents (with a frequency of 31.3%) and in the cecal contents (25-37.5%) of pigs fed on SL non-GM maize. These results suggested that ingested DNA was not totally degraded, but is present in a form detectable by PCR.
Keywords: genetically modified (GM) StarLink CBH351 maize (SL), cry9C gene, zein (Zel) gene, DNA degredation, intestinal contents
Citation:
Chowdhury, E.H., Mikami, O., Nakajima, Y., Hino, A., Kuribara, H., Suga, K., Hanazumi, M. and Yomemochi, C., 2003. Detection of genetically modified maize DNA fragments in the intestinal contents of pigs fed StarLink CBH351. Veterinary and Human Toxicology, 45(2), 95-96.
Category:
- Health effects
- Pesticide use
Record ID: 375
