Journal or Publishing Institution: Food Chemistry
Study: http://www.sciencedirect.com/science/article/pii/S0308814613012570
Author(s): Nikolić, Z., Vasiljević, I., Zdjelar, G., Đorđević, V., Ignjatov, M., Jovičić, D. and Milošević, D.
Article Type: Peer Reviewed Study
Record ID: 1733
Abstract: In order to detect presence and quantity of Roundup Ready (RR) soybean in crude oil extracted from soybean seed with a different percentage of GMO seed two extraction methods were used, CTAB and DNeasy Plant Mini Kit. The amplifications of lectin gene, used to check the presence of soybean DNA, were not achieved in all CTAB extracts of DNA, while commercial kit gave satisfactory results. Comparing actual and estimated GMO content between two extraction methods, root mean square deviation for kit is 0.208 and for CTAB is 2.127, clearly demonstrated superiority of kit over CTAB extraction. The results of quantification evidently showed that if the oil samples originate from soybean seed with varying percentage of RR, it is possible to monitor the GMO content at the first stage of processing crude oil.
Keywords: GMO, Soybean, Crude Oil; Agricultural Crops, Plant DNA, Drug Resistance, Food Contamination, Food Handling, Food Inspection, Food Labeling, Glycine, Herbicides, Plant Lectins, Genetically Modified Plants, Polymerase Chain Reaction, Real-Time Polymerase Chain Reaction, Reproducibility of Results, Seeds, Serbia, Soybean Oil, Soybean Proteins, Soybeans; Chemistry, Drug Effects, Genetics, Metabolism, Methods, Legislation & Jurisprudence; DNA, Genes, Genetically Modified Organisms, Lectins, Monitoring, Sampling
Citation: Nikolić, Z., Vasiljević, I., Zdjelar, G., Đorđević, V., Ignjatov, M., Jovičić, D. and Milošević, D., 2014. Detection of genetically modified soybean in crude soybean oil. Food Chemistry, 145, pp.1072-1075.
