Journal or Publishing Institution: Aquaculture Research
Author(s): Ran, T., Mei, L., Lei, W., Aihua, L., Ru, H. and Jie, S.
Article Type: Peer Reviewed Study
Record ID: 2028
Abstract: We used nested‐polymerase chain reaction (PCR) to detect Roundup Ready soybean in aquatic feeds and feeding tilapias. A template concentration of 10−10 g μL−1 DNA solution could be detected with a dilute degree of 0.01%. Most (90.6%) of the aquatic feeds containing soybean byproduct included exogenous DNA segments. We also compared genetically modified (GM) soybean with non‐GM soybean diets in feeding tilapias (Oreochromis niloticus, GIFT strain) and examined the residual fragments (254 bp) of GM soybeans. Tilapias receiving GM soybean diets had DNA fragments in different tissues and organs, indicating that exogenous GM genes were absorbed systemically and not completely degraded by the tilapia’s alimentary canal.
Keywords: nested-PCR, Oreochromis niloticus, transfer, GM soybean, tissues, aquatic feed, Glycine (Fabaceae), Oreochromis, aquatic animals, aquatic organisms, byproducts, detection, diets, DNA, feeding, feeds, genes, soyabeans, techniques, transgenics, aquatic species, deoxyribonucleic acid, feeding stuffs, Nile tilapia, soybeans
Citation: Ran, T., Mei, L., Lei, W., Aihua, L., Ru, H. and Jie, S., 2009. Detection of transgenic DNA in tilapias (Oreochromis niloticus, GIFT strain) fed genetically modified soybeans (Roundup Ready). Aquaculture Research, 40(12), pp.1350-1357.
