Journal or Publishing Institution: Environmental Toxicology and Pharmacology
Study: http://www.sciencedirect.com/science/article/pii/S1382668913001336
Author(s): Nwani, C.D., Nagpure, N.S., Kumar, R., Kushwaha, B. and Lakra, W.S.
Article Type: Peer Reviewed Study
Record ID: 1795
Abstract: The present study was undertaken to evaluate the genotoxic and oxidative stress modulatory effects of commercial formulation of glyphosate-based herbicide (Roundup®) in freshwater fish Channa punctatus. Three sublethal test concentrations of the herbicide viz., SL-I (1/10th of LC50 = ∼3.25 mg L−1), SL-II (1/8th of LC50 = ∼4.07 mg L−1) and SL-III (1/5th of LC50 = ∼6.51 mg L−1) were calculated using 96-LC50 value and the test specimens were exposed to these concentrations. Blood and gill cells of the exposed specimens were sampled on day 1, 7, 14, 21, 28 and 35 to examine the DNA damage using comet assay and to assess the alteration in lipid peroxidation and antioxidant enzymes activities. The highest DNA damage was observed on day 14 at all test concentrations followed by gradual non-linear decline. Induction of oxidative stress in the blood and gill cells were evidenced by increased lipid peroxidation level, while antioxidants namely superoxide dismutase, catalase and glutathione reductase responded in a concentration-dependent manner. The results supported the integrated use of comet and antioxidant assays in determining the toxicity of water pollutants which could be used as part of monitoring programs.
Keywords: Roundup, Channa punctatus, Comet Assay, DNA Damage, Antioxidant Enzymes, Oxidative Stress; Animals, Biomarkers, Catalase, Drug Dose-Response Relationship, Environmental Monitoring, Fresh Water, Gills, Glutathione Reductase, Glycine, Herbicides, Lipid Peroxidation, Perciformes, Superoxide Dismutase, Time Factors, Chemical Water Pollutants; Metabolism, Methods, Analysis, Drug Effects, Pathology, Analogs & Derivatives, Toxicity, Blood
Citation: Nwani, C.D., Nagpure, N.S., Kumar, R., Kushwaha, B. and Lakra, W.S., 2013. DNA damage and oxidative stress modulatory effects of glyphosate-based herbicide in freshwater fish, Channa punctatus. Environmental Toxicology and Pharmacology, 36(2), pp.539-547.
