Evaluation of the genotoxic and cytotoxic effects of glyphosate-based herbicides in the ten spotted live-bearer fish Cnesterodon decemmaculatus (Jenyns, 1842)

Journal or Publishing Institution: Ecotoxicology and Environmental Safety

Study: http://www.sciencedirect.com/science/article/pii/S0147651312004484

Author(s): Vera-Candioti, J., Soloneski, S. and Larramendy, M.L.

Article Type: Peer Reviewed Study

Record ID: 869

Abstract: Mortality, genotoxicity, and cytotoxicity of the 48% glyphosate-based formulations Panzer and Credit^® were evaluated on Cnesterodon decemmaculatus (Jenyns, 1842) (Pisces, Poeciliidae) under laboratory conditions. Induction of micronuclei (MN) and alterations in the erythrocytes: erythroblasts ratio were employed as end points for genotoxicity and cytotoxicity, respectively. For Panzer^®, mean values of 16.70 and 15.68 mg/L were determined for LC₅₀ at 24 and 96 h, respectively, and these concentrations reached mean values of 98.50 and 91.73 mg/L for Credit^®. LC₅₀ values decreased as a negative linear function of Panzer^® exposure time within the 0–96 h period, but not for Credit^®. LC₅₀ values indicated that the fish were more sensitive to Panzer^® than to Credit^®. Both 3.9 and 7.8 mg/L of Panzer^®increased MN frequency at 48 and 96 h of treatment. When fish were exposed to Credit^®, an increased frequency of MN over control values was found after 96 h for all concentrations assayed, but not after 48 h. No cellular cytotoxicity was found after Panzer^® and Credit^®treatment, regardless of both the concentration and the sampling time. Furthermore, our results demonstrated that Panzer^® and Credit^®should be considered as glyphosate-based commercial formulations with genotoxic but not cytotoxic effect properties.

Keywords: Mortality, Micronucleus, Erythrocyte:erythroblast ratio, Poeciliidae, Panzer, Credit, Fish, Cnesterodon decemmaculatus, Glyphosate, Ten spotted live-bearer, Genotoxic and Cytotoxic

Citation: Vera-Candioti, J., Soloneski, S. and Larramendy, M.L., 2013. Evaluation of the genotoxic and cytotoxic effects of glyphosate-based herbicides in the ten spotted live-bearer fish Cnesterodon decemmaculatus (Jenyns, 1842). Ecotoxicology and Environmental Safety, 89, pp.166-173.