Journal or Publishing Institution: Cell Research
Study: http://www.ncbi.nlm.nih.gov/pubmed/21931358
Author(s): Zhang, L., Hou, D., Chen, X., Li, D., Zhu, L., Zhang, Y., Li, J., Bian, Z., Liang, X., Cai, X. and Yin, Y.,
Article Type: Peer Reviewed Study
Record ID: 1744
Abstract: Our previous studies have demonstrated that stable microRNAs (miRNAs) in mammalian serum and plasma are actively secreted from tissues and cells and can serve as a novel class of biomarkers for diseases, and act as signaling molecules in intercellular communication. Here, we report the surprising finding that exogenous plant miRNAs are present in the sera and tissues of various animals and that these exogenous plant miRNAs are primarily acquired orally, through food intake. MIR168a is abundant in rice and is one of the most highly enriched exogenous plant miRNAs in the sera of Chinese subjects. Functional studies in vitro and in vivo demonstrated that MIR168a could bind to the human/mouse low-density lipoprotein receptor adapter protein 1 (LDLRAP1) mRNA, inhibit LDLRAP1 expression in liver, and consequently decrease LDL removal from mouse plasma. These findings demonstrate that exogenous plant miRNAs in food can regulate the expression of target genes in mammals.
Keywords: LDLRAP1, AGO2, Luciferase, Cells, Antibody (Antibodies), Binding, Membrane, Mouse, Rice (Oryza sativa), Cancer, Atheroscelorsis, Carcinoma, Hepatoma, Protein, Cholesterol, Oligonucleotides, Periodate, Diet, Caco-2, HepG2; Gene Expression, Interspecies Regulation, Mammals, Mirnas, Plant; MicroRNA, MIR168a, LDLRAP1, Low-Density Lipoprotein, Microvesicle, Cross-Kingdom
Citation: Zhang, L., Hou, D., Chen, X., Li, D., Zhu, L., Zhang, Y., Li, J., Bian, Z., Liang, X., Cai, X. and Yin, Y., 2012. Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA. Cell Research, 22(1), p.107.
