Journal or Publishing Institution: Reproductive Toxicology
Author(s): Alves, M.G., Neuhaus-Oliveira, A., Moreira, P.I., Socorro, S. and Oliveira, P.F.
Article Type: Peer Reviewed Study
The purpose of this study was to determine the effects of 2,4-D, an herbicide used worldwide also known as endocrine disruptor, in Sertoli cell (SC) metabolism.
Immature rat SCs were maintained 50 h under basal conditions or exposed to 2,4-D (100 nM, 10 μM and 1 mM). SCs exposed to 10 μM and 1 mM of 2,4-D presented lower intracellular glucose and lactate content. Exposure to 10 μM of 2,4-D induced a significant decrease in glucose transporter-3 mRNA levels and phosphofructokinase-1 mRNA levels decreased in cells exposed to 100 nM and 10 μM of 2,4-D. Exposure to 100 nM and 10 μM also induced a decrease in lactate dehydrogenase (LDH) mRNA levels while the LDH protein levels were only decreased in cells exposed to 1 mM of 2,4-D.
Exposure to 2,4-D altered glucose uptake and metabolization in SCs, as well as lactate metabolism and export that may result in impaired spermatogenesis.
Keywords: 2, 4-dichlorophenoxyacetic acid, glucose metabolism, rat, Sertoli cells, rodent
Alves, M.G., Neuhaus-Oliveira, A., Moreira, P.I., Socorro, S. and Oliveira, P.F., 2013. Exposure to 2, 4-dichlorophenoxyacetic acid alters glucose metabolism in immature rat Sertoli cells. Reproductive Toxicology, 38, pp.81-88.