Journal or Publishing Institution: Toxicology in Vitro
Study: https://www.sciencedirect.com/science/article/abs/pii/S0887233306000798
Author(s): González, N.V., Soloneski, S. and Larramendy, M.L.
Article Type: Peer Reviewed Study
Abstract:
The cytogenetic effects exerted by the phenoxy herbicide dicamba and one of its commercial formulations banvel® (57.71% dicamba) were studied in in vitro whole blood human lymphocyte cultures. The genotoxicity of herbicides was measured by analysis of the frequency of sister chromatid exchanges (SCEs) and cell-cycle progression assays. Both dicamba and banvel® activities were tested within 10.0–500.0 μg/ml doses range. Only concentrations of 200.0 μg/ml of dicamba and 500.0 μg/ml of banvel® induced a significant increase in SCE frequency over control values. The highest dose of dicamba tested (500.0 μg/ml) resulted in cell culture cytotoxicity. The cell-cycle kinetics was affected by both test compounds since a significant delay in cell-cycle progression and a significant reduction of the proliferative rate index were observed after the treatment with 100.0 and 200.0 μg/ml of dicamba and 200.0 and 500.0 μg/ml of banvel®. For both chemicals, a progressive dose-related inhibition of the mitotic activity of cultures was observed. Moreover, only the mitotic activity statistically differed from control values when doses of both chemicals higher than 100.0 μg/ml were employed. On the basis of our results, the herbicide dicamba is a DNA damage agent and should be considered as a potentially hazardous compound to humans.
Keywords: Dicamba, Banvel, Chlorophenoxy herbicides, Sister chromatid exchanges, Cell-cycle kinetics
Citation:
González, N.V., Soloneski, S. and Larramendy, M.L., 2006. Genotoxicity analysis of the phenoxy herbicide dicamba in mammalian cells in vitro. Toxicology in Vitro, 20(8), pp.1481-1487.
