Journal or Publishing Institution: Ecotoxicology and Environmental Safety
Study: https://www.ncbi.nlm.nih.gov/pubmed/19013644
Author(s): Mañas, F., Peralta, L., Raviolo, J., Ovando, H.G., Weyers, A., Ugnia, L., Cid, M.G., Larripa, I. and Gorla, N.
Article Type: Peer Reviewed Study
Record ID: 1500
Abstract: Formulations containing glyphosate are the most widely used herbicides in the world. AMPA is the major environmental breakdown product of glyphosate. The purpose of this study is to evaluate the in vitro genotoxicity of AMPA using the Comet assay in Hep-2 cells after 4h of incubation and the chromosome aberration (CA) test in human lymphocytes after 48h of exposition. Potential in vivo genotoxicity was evaluated through the micronucleus test in mice. In the Comet assay, the level of DNA damage in exposed cells at 2.5-7.5mM showed a significant increase compared with the control group. In human lymphocytes we found statistically significant clastogenic effect AMPA at 1.8mM compared with the control group. In vivo, the micronucleus test rendered significant statistical increases at 200-400mg/kg. AMPA was genotoxic in the three performed tests. Very scarce data are available about AMPA potential genotoxicity.
Keywords: Herbicide, DNA Damage, Chromosome Damage, Glyphosate, AMPA; Assessment, Cytogenetic Techniques, Environmental Degradation, Genotoxicity, Glyphosate, Herbicide Residues, Herbicides, Metabolites, Public Health; Adolescent, Adult, Animals, Hepatocellular Carcinoma, Tumor Cell Line, Cell Survival, Chromosome Aberration, DNA, DNA Damage, Environmental Pollutants, Female, Glycine, Herbicides, Humans, Isoxazoles, Lymphocytes, Male, Mice, Inbred BALB C Mice, Mutagenicity Tests, Mutagens, Organophosphonates, Tetrazoles, Young Adults; Drug Effects, Toxicity, Metabolism, Pathology, Methods
Citation: Mañas, F., Peralta, L., Raviolo, J., Ovando, H.G., Weyers, A., Ugnia, L., Cid, M.G., Larripa, I. and Gorla, N., 2009. Genotoxicity of AMPA, the environmental metabolite of glyphosate, assessed by the Comet assay and cytogenetic tests. Ecotoxicology and Environmental Safety, 72(3), pp.834-837.
