Journal or Publishing Institution: BMC Genomics
Study: http://link.springer.com/article/10.1186%2Fs12864-015-1254-5
Author(s): Webster, T.M.U. and Santos, E.M.
Article Type: Peer Reviewed Study
Record ID: 1052
Abstract: Glyphosate, the active ingredient in Roundup formulations, is the most widely used herbicide worldwide, and as a result contaminates surface waters and has been detected in food residues, drinking water and human urine, raising concerns for potential environmental and human health impacts. Research has shown that glyphosate and Roundup can induce a broad range of biological effects in exposed organisms, particularly via generation of oxidative stress. However, there has been no comprehensive investigation of the global molecular mechanisms of toxicity of glyphosate and Roundup for any species. We aimed to characterise and compare the global mechanisms of toxicity of glyphosate and Roundup in the liver of brown trout (Salmo trutta), an ecologically and economically important vertebrate species, using RNA-seq on an Illumina HiSeq 2500 platform. To do this, we exposed juvenile female brown trout to 0, 0.01, 0.5 and 10 mg/L of glyphosate and Roundup (glyphosate acid equivalent) for 14 days, and sequenced 6 replicate liver samples from each treatment.
Keywords: RNA-seq, Transcriptome, Herbicide, Salmonids, Toxicogenomics
Citation: Webster, T.M.U. and Santos, E.M., 2015. Global transcriptomic profiling demonstrates induction of oxidative stress and of compensatory cellular stress responses in brown trout exposed to glyphosate and Roundup. BMC Genomics, 16(1), p.32.
