Herbicide 2,4-dichlorophenoxyacetic acid (2,4-D)-induced cytogenetic damage in human lymphocytes in vitro in presence of erythrocytes

Journal or Publishing Institution: Cell Biology International

Study: http://www.ncbi.nlm.nih.gov/pubmed/17606385

Author(s): Soloneski, S., González, N.V., Reigosa, M.A. and Larramendy, M.L.

Article Type: Peer Reviewed Study

Record ID: 2181

Abstract: The genotoxic effects of 2,4-D and its commercial derivative 2,4-D DMA were studied by measuring sister chromatid exchange (SCE), cell-cycle progression and mitotic index in human whole blood (WBC) and plasma leukocyte cultures (PLC). Concentrations of 10, 25, 50 and 100 microg herbicide/ml were used during 72 h. In WBC, a significant increase in SCE frequency was observed within the 10-50 microg 2,4-D/ml and 25-100 microg 2,4-D DMA/ml dose range. Contrarily, in PLC, none of the concentrations employed affected the SCEs frequency. A significant delay in cell proliferation was observed in WBC after treatments with 25 and 50 microg 2,4-D/ml and 50 and 100 microg 2,4-D DMA/ml. In PLC, only 100.0 microg 2,4-D/ml altered cell-cycle progression. For both chemicals, a progressive dose-related inhibition of mitotic activity was observed. The results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4-D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMA were more potent genotoxic agents in the presence of human red cells.

Keywords: 2,4-D, 2,4-D DMA, Human Lymphocytes, Sister Chromatid Exchanges, Cell-Cycle Progression, Mitotic Activity, 2,4-Dichlorophenoxyacetic Acid, Adult, Cell Cycle, Cultured Cells, Erythrocytes, Herbicides, Humans, Lymphocytes, Male, Mutagenicity Tests, Sister Chromatid Exchange, Metabolism, Pharmacology, Drug Effects, Cytology,

Citation: Soloneski, S., González, N.V., Reigosa, M.A. and Larramendy, M.L., 2007. Herbicide 2, 4‐dichlorophenoxyacetic acid (2, 4‐D)‐induced cytogenetic damage in human lymphocytes in vitro in presence of erythrocytes. Cell Biology International, 31(11), pp.1316-1322.