Journal or Publishing Institution: Aquatic Toxicology
Study: http://www.sciencedirect.com/science/article/pii/S0166445X08002257
Author(s): Stachowski-Haberkorn, S., Becker, B., Marie, D., Haberkorn, H., Coroller, L. and De La Broise, D.
Article Type: Peer Reviewed Study
Record ID: 2202
Abstract: The effects of the herbicide Roundup® (glyphosate) on natural marine microbial communities were assessed in a 7-day field experiment using microcosms. Bottles were maintained underwater at 6 m depth, and 10% of their water content was changed every other day.
The comparison of control microcosms and surrounding surface water showed that the microcosm system tested here can be considered as representative of the natural surrounding environment. A temporal temperature gradient gel electrophoresis (TTGE) was run on 16S and 18S rDNA-amplified extracts from the whole microbial community. Cluster analysis of the 16S gel showed differences between control and treatment fingerprints for Roundup at 1 μg L−1 (ANOSIM, p = 0.055; R = 0.53), and 10 μg L−1 (ANOSIM, p = 0.086; R = 0.40). Flow cytometry analysis revealed a significant increase in the prasinophyte-like population when Roundup concentration was increased to 10 μg L−1.
This study demonstrates that a disturbance was caused to the marine microbial community exposed to 1 μg L−1 Roundup concentration, a value typical of those reported in coastal waters during a run-off event.
Keywords: Microcosm, Marine Microbial Communities, Glyphosate, TTGE, Flow Cytometry, glyphosate, water pollution, coastal water, seawater, microorganisms, prokaryotic cells, eukaryotic cells, phytoplankton, community structure, toxicity, chlorophyll, France, Animals, Chlorophyll A, DNA Fingerprinting, Diatoms, Dinoflagellida, Polyacrylamide Gel Electrophoresis, Glycine, Herbicides, Polymerase Chain Reaction, Synechococcus, Water Microbiology, Chemical Water Pollutants, Analysis, Metabolism, Methods, Drug Effects, Growth & Development, Toxicity
Citation: Stachowski-Haberkorn, S., Becker, B., Marie, D., Haberkorn, H., Coroller, L. and De La Broise, D., 2008. Impact of Roundup on the marine microbial community, as shown by an in situ microcosm experiment. Aquatic Toxicology, 89(4), pp.232-241.
