In vitro cytotoxicity assessment of roundup (glyphosate) in L-02 hepatocytes

Journal or Publishing Institution: Journal of Environmental Science and Health

Study: https://www.tandfonline.com/doi/full/10.1080/03601234.2017.1293449?casa_token=-vg8TLWHVEwAAAAA:IF2HThpGY5pERJQa8LgqxWHCklrLS8TLqu4V_QKZMIdjPUVMItcF5eUFzOqbnbv1dlOoLtitYOse

Author(s): Luo, L., Wang, F., Zhang, Y., Zeng, M., Zhong, C. and Xiao, F.

Article Type: Peer Reviewed Study

Record ID: 1469

Abstract: The goal of the present study was to elucidate the in vitro cytotoxicity of Roundup and to reveal the possible related mechanisms in L-02 hepatocytes. By detecting reactive oxygen species (ROS) production, glutathione (GSH)/superoxide dismutase (SOD) levels, mitochondrial permeability transition pore (PTP) open rate, apoptosis-inducing factor (AIF) release, intracellular Ca²⁺ concentration, and alanine aminotransferease (ALT)/aspartate aminotransferase (AST) leakage, we determined that Roundup induced anti-oxidant system inhibition, mitochondria damage, DNA damage, membrane integrity and permeability changes, and apoptosis in L-02 hepatocytes. By revealing the mechanistic insights of Roundup-induced cytotoxicity, our results are valuable for the design of preventive and therapeutic strategies for the occupational population exposed to Roundup and other pesticides.

Keywords: Roundup, Glyphosate, Cytotoxicity, ROS, L-02 Hepatocytes; DNA Damage, Alanine Transaminase, Antioxidants, Apoptosis, Aspartate Tranaminase, Calcium, Cytotoxicity, Glutathione, Mitochondria, Permeability, Reactive Oxygen Species, Superoxide Dismutase, Toxicity Tests, Reactive Oxygen Species, Glutathione Peroxidase, Aspartate Aminotransaminase, Glycine; Metabolism, Drug Effects, Toxicity, Physiology, Methods, Superoxide Dismutase, ROS,

Citation: Luo, L., Wang, F., Zhang, Y., Zeng, M., Zhong, C. and Xiao, F., 2017. In vitro cytotoxicity assessment of roundup (glyphosate) in L-02 hepatocytes. Journal of Environmental Science and Health, Part B, 52(6), pp.410-417.