Journal or Publishing Institution: Springer
Study: http://link.springer.com/chapter/10.1007/978-3-642-23271-8_33
Author(s): Tudisco, R., Calabro, S., Cutrignelli, M.I., Grossi, M., Piccolo, V. and Infascelli, F.
Article Type: Peer Reviewed Study
Record ID: 2019
Abstract: PCR approach was used in order to investigate the presence of plant DNA in pellet of somatic cells from fresh or stored milk of twenty grazing goats fed concentrate (18% CP; 1.03 UFL/kg, as fed) containing conventional or genetically modified soybean meal. The quantity and the purity of DNAs extracted from fresh samples were higher than those of samples stored at −20°C (ng/μl: 51.6 vs 20.8; A260/A280: 1.7 vs 1.4). The frequency of chloroplast sequence, for both groups, ranged between 70 and 100% in fresh and between 30 and 40% in stored samples. The frequency percentage of specific soybean gene was lower in stored (25–33%) than in fresh samples (56–88%). Transgenic fragments were found only in milk from goats fed GM soybean. For 35S promoter, the frequency was 38–75% in fresh and 25% in stored samples, while for CP4 EPSPS, it was 38–50% in fresh and 25% in stored samples.
Keywords: Milk, Goat, DNA extraction, Transgene
Citation: Tudisco, R., Calabro, S., Cutrignelli, M.I., Grossi, M., Piccolo, V. and Infascelli, F., 2012. Influence of Sample Storage on the Quality of DNA Extracted from Milk of Goats Fed Conventional or Transgenic Soybean. In Veterinary Science (pp. 199-203). Berlin, Heidelberg: Springer.
