Journal or Publishing Institution: Plants, People, Planet
Study: https://nph.onlinelibrary.wiley.com/doi/full/10.1002/ppp3.10156
Author(s): Shimono, A., Kanbe, H., Nakamura, S., Ueno, S., Yamashita, J. and Asai, M.
Article Type: Peer Reviewed Study
Abstract:
Summary
- The US is the largest source to Japan of crops genetically modified to be glyphosate resistant (GR). The intensive use of glyphosate in the US has led to the evolution of GR Amaranthus palmeri, one of the most problematic weeds in the US. Here, we investigated the initial invasion and establishment of GR A. palmeri at grain-importing ports in Japan.
- The primary glyphosate resistance mechanism is a copy-number amplification of the 297-kb region containing the herbicide target site gene 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). We used quantitative PCR to measure the EPSPS genomic copy number and used PCR to confirm the presence of the other amplified region. We used microsatellite marker analysis to compare the genetic similarities between Japanese populations and US accessions.
- We detected GR A. palmeri at three ports: although present as a casual plant at two of the three ports, GR populations were established at one of the ports investigated. The port populations were found to be genetically similar to the US accessions and showed no geographical genetic structure.
- This study shows that GR A. palmeri has naturalized in Japan in less than 10 years from the first report of GR A. palmeri in the US.
Keywords: gene amplification, glyphosate-resistant, herbicide, introduced species, invasion, Palmer amaranth, seed contaminant
Citation:
Shimono, A., Kanbe, H., Nakamura, S., Ueno, S., Yamashita, J. and Asai, M., 2020. Initial invasion of glyphosate‐resistant Amaranthus palmeri around grain‐import ports in Japan. Plants, People, Planet, 2(6), pp.640-648.
Record ID: 2660