Intranasal, rectal and intraperitoneal immunization with protoxin Cry1Ac from Bacillus thuringiensis induces compartmentalized serum, intestinal, vaginal and pulmonary immune responses in Balb/c mice

Journal or Publishing Institution: Microbes and Infection

Study: http://www.ncbi.nlm.nih.gov/pubmed/10962271

Author(s): Moreno-Fierros, L., García, N., Gutiérrez, R., López-Revilla, R. and Vázquez-Padrón, R.I.

Article Type: Peer Reviewed Study

Record ID: 1672

Abstract: Recently we discovered that the Cry1Ac protoxin of Bacillus thuringiensis administered to Balb/c mice intraperitoneally (i.p.) or intragastrically is a systemic and intestinal immunogen as potent as cholera toxin. To further characterize the mucosal immunogenicity of Cry1Ac we additionally tried the intranasal (i.n.) and rectal routes and used enzyme-linked immunoassays to determine anti-Cry1Ac antibody responses in the serum as well as in vaginal and tracheobronchial washes and in the fluids of the large and the small intestine. Immunization by the i.p., i.n. and rectal routes induced IgM, IgG and IgA antibodies in all the mucosal surfaces analyzed, but the magnitude and predominant isotype of each response depended on the route used and the mucosal site analyzed. These data extend our findings on the striking mucosal immunogencity of Cry1Ac and provide additional evidence on the compartmentalization of the mucosal immune system.

Keywords: Cry1Ac Protoxin, Mucosal Immune Compartmentalization, Vaginal Immunity, Mucosal Immunity; Cholera Toxin, Large Intestine, Immunoenzyme Techniques, Immunoglobluin A, Small Intestine, Antibody Formation, Immunoglobulin M, Anti-Idiotypic Antibodies, Immune System, Immunization, Immunoglobulin G, Lung, Antibodies, Serum, Bacillus Thuringiensis Protoxin; Microbiology, Infectious Diseases; Intranasal Administration, Animals, Bacillus thuringiensis, Bacterial Proteins, Bacterial Toxins, Endotoxins, Female, Hemolysin Proteins, Mucosal Immunity, Intraperitoneal Injections, Large Intestine, Small Intestine, Mice, Inbred BALB C Mice, Protein Precursors, Rectum, Respiratory System, Vaccination, Vagina; Blood, Immunology, Methods; Antibodies, Antigens, Bacterial Toxins, Bronchi, IgA, IgG, IgM, Immune Response, Immune Serum, Immunization, Laboratory Animals, Mucosa, Small Intestine, Trachea; Antigenicity, Antiserum, Bacterium, Immune Sensitization, Immunity Reactions, Immunogens, Immunological Reactions, Mucous Membrane

Citation: Moreno-Fierros, L., García, N., Gutiérrez, R., López-Revilla, R. and Vázquez-Padrón, R.I., 2000. Intranasal, rectal and intraperitoneal immunization with protoxin Cry1Ac from Bacillus thuringiensis induces compartmentalized serum, intestinal, vaginal and pulmonary immune responses in Balb/c mice. Microbes and Infection, 2(8), pp.885-890.