New insights on proteomics of transgenic soybean seeds: Evaluation of differential expressions of enzymes and proteins

Journal or Publishing Institution: Analytical and Bioanalytical Chemistry

Date of Publication: 01/01/2012

Study: https://www.researchgate.net/profile/Marco_Arruda2/publication/51674088_New_insights_on_proteomics_of_transgenic_soybean_seeds_Evaluation_of_differential_expressions_of_enzymes_and_proteins/links/02e7e52226f36ea2e0000000.pdf

Author(s): Barbosa, H.S., Arruda, S.C., Azevedo, R.A., and Arruda, M.A.

Article Type: Peer Reviewed Study

Abstract:

This work reports the evaluation of differentially expressed enzymes and proteins from transgenic and nontransgenic soybean seeds. Analysis of malondialdehyde, ascorbate peroxidase (EC 1.11.1.11), glutathione reductase (EC 1.6.4.2), and catalase (EC 1.11.1.6) revealed higher levels (29.8, 30.6, 71.4, and 35.3%, respectively) in transgenic seeds than in nontransgenic seeds. Separation of soybean seed proteins was done by two-dimensional polyacrylamide gel electrophoresis, and 192 proteins were identified by matrix-assisted laser desorption/ionization (MALDI) quadrupole time-of-flight (QTOF) mass spectrometry (MS) and electrospray ionization (ESI) QTOF MS. Additionally, the enzyme CP4 EPSPS, involved in the genetic modification, was identified by enzymatic digestions using either trypsin or chymotrypsin and ESI-QTOF MS/MS for identification. From the proteins identified, actin fragment, cytosolic glutamine synthetase, glycinin subunit G1, and glycine-rich RNA-binding protein were shown to be differentially expressed after analysis using the two-dimensionaldifferencegelelectrophoresistechnique,and applying a regulator factor of 1.5 or greater.

Keywords: two-dimensional difference gel electrophoresis, mass spectrometry, reactive oxygen species, genetically modified (GM) soybean, genetically engineered (GE), Roundup Ready soybean, glyphosate tolerant soybean, transgenic crops, GMO, substantial equivalence

Citation:

Barbosa, H.S., Arruda, S.C., Azevedo, R.A., & Arruda, M.A., 2012. New insights on proteomics of transgenic soybean seeds: evaluation of differential expressions of enzymes and proteins. Analytical and Bioanalytical Chemistry, 402(1), 299-314.

Category:

• Environmental effects

Record ID: 133