Journal or Publishing Institution: Applied Environmental Microbiology
Date of Publication: 03/01/1989
Study: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC184185/pdf/aem00096-0177.pdf
Author(s): Bartsch, K. and Tebbe, C.
Article Type: Peer Reviewed Study
Abstract:
Three hundred bacterial isolates from soil were tested for resistance against phosphinothricin [PPT; DL-homoalanin-4-yl(methyl)phosphinic acid], the active ingredient of the herbicide BASTA. Eight resistant bacterial strains and Escherichia coli were analyzed for PPT-transforming activities. At least three different enzymatic reactions could be detected in cell extracts. In six strains an acetyltransferase was active, synthesizing N-acetyl-PPT in the presence of PPT and acetyl coenzyme A. All strains could degrade PPT to its corresponding 2-oxoacid {2-oxo-4-[(hydroxy)(methyl)phosphinoyl] butyric acid} by transamination. Rhodococcus sp., the only tested strain that was able to utilize PPT as a sole source of nitrogen, formed 2oxo-4[(hydroxy)(methyl)phosphinoyl] butyric acid by oxidative deamination. This enzymatic activity was inducible by L-glutamic acid or PPT itself but not in the presence of NH4′. D-PPT transformation was not detectable in any of the investigated strains.
Keywords: glufosinate, BASTA, phosphinothricin, Escherichia coli, herbicide resistance, soil microbiome, soil microbiota, soil microflora, bacteria, pesticides, herbicides
Citation:
Bartsch, K. and Tebbe, C., 1989. Initial steps in the degradation of phosphinothricin (glufosinate) by soil bacteria. Applied Environmental Microbiology, 55, pp 711-716.
Category:
- Environmental effects
- Pesticide use
Record ID: 141
