Journal or Publishing Institution: Chemical Research in Toxicology
Study: http://pubs.acs.org/doi/abs/10.1021/tx015543g
Author(s): Marc, J., Mulner-Lorillon, O., Boulben, S., Hureau, D., Durand, G. and Bellé, R.
Article Type: Peer Reviewed Study
Record ID: 1504
Abstract: To assess human health risk from environmental chemicals, we have studied the effect on cell cycle regulation of the widely used glyphosate-containing pesticide Roundup. As a model system we have used sea urchin embryonic first divisions following fertilization, which are appropriate for the study of universal cell cycle regulation without interference with transcription. We show that 0.8% Roundup (containing 8 mM glyphosate) induces a delay in the kinetic of the first cell cleavage of sea urchin embryos. The delay is dependent on the concentration of Roundup. The delay in the cell cycle could be induced using increasing glyphosate concentrations (1−10 mM) in the presence of a subthreshold concentration of Roundup 0.2%, while glyphosate alone was ineffective, thus indicating synergy between glyphosate and Roundup formulation products. The effect of Roundup was not lethal and involved a delay in entry into M-phase of the cell cycle, as judged cytologically. Since CDK1/cyclin B regulates universally the M-phase of the cell cycle, we analyzed CDK1/cyclin B activation during the first division of early development. Roundup delayed the activation of CDK1/cyclin B in vivo. Roundup inhibited also the global protein synthetic rate without preventing the accumulation of cyclin B. In summary, Roundup affects cell cycle regulation by delaying activation of the CDK1/cyclin B complex, by synergic effect of glyphosate and formulation products. Considering the universality among species of the CDK1/cyclin B regulator, our results question the safety of glyphosate and Roundup on human health.
Keywords: Animals, CDC2 Protein Kinase, Cell Cycle, Cycle Division, Cyclin B, Nonmammalian Embryo, Enzyme Activation, Glycine, Herbicides, Ovum, Sea Urchins, Time Factors; Cdk1 Cyclin Activation, Division Dysfunction, Cell Cycle, Pesticide Roundup Provokes, Cell Cycle Regulation, Cdk1 Cyclin, Sea Urchin Embryonic First Division, Glyphosate-Containing Pesticide Roundup, Mm Glyphosate, Subthreshold, Concentration, Roundup Formulation Product, 1-10mm, Early Development, Global Protein Synthetic Rate, Cdk1 Cyclin Complex, Formulation Product, Human Health, First Division, Sea Urchin Embryo, Cdk1 Cyclin Regulator, Universal Cell Cycle Regulation, First Cell Cleavage, Human Health Risk, Model System, Glyphosate Concentration, Environmental Chemical, Synergic Effect
Citation: Marc, J., Mulner-Lorillon, O., Boulben, S., Hureau, D., Durand, G. and Bellé, R., 2002. Pesticide Roundup provokes cell division dysfunction at the level of CDK1/cyclin B activation. Chemical Research in Toxicology, 15(3), pp.326-331.