Journal or Publishing Institution: Pest Management Science
Study: http://onlinelibrary.wiley.com/doi/10.1002/ps.1766/abstract
Author(s): Song, X., Liu, L., Wang, Z. and Qiang, S.
Article Type: Peer Reviewed Study
Record ID: 2185
Abstract:
BACKGROUND: The possibility of gene flow from transgenic crops to wild relatives may be affected by reproductive capacity between them. The potential gene flow from two transgenic rice lines containing the bar gene to five accessions of weedy rice (WR1–WR5) was determined through examination of reproductive compatibility under controlled pollination.
RESULTS: The pollen grain germination of two transgenic rice lines on the stigma of all weedy rice, rice pollen tube growth down the style and entry into the weedy rice ovary were similar to self‐pollination in weedy rice. However, delayed double fertilisation and embryo abortion in crosses between WR2 and Y0003 were observed. Seed sets between transgenic rice lines and weedy rice varied from 8 to 76%. Although repeated pollination increased seed set significantly, the rank of the seed set between the weedy rice accessions and rice lines was not changed. The germination rates of F1 hybrids were similar or greater compared with respective females. All F1 plants expressed glufosinate resistance in the presence of glufosinate selection pressure.
CONCLUSIONS: The frequency of gene flow between different weedy rice accessions and transgenic herbicide‐resistant rice may differ owing to different reproductive compatibility. This result suggests that, when wild relatives are selected as experimental materials for assessing the gene flow of transgenic rice, it is necessary to address the compatibility between transgenic rice and wild relatives.
Keywords: Oryza, Oryza sativa, plants, abortion (plants), compatibility, crosses, fertilization, gene flow, genetic transformation, genetically engineered organisms, glufosinate, glufosinate-P, herbicide resistance, herbicides, hybrids, plant embryos, pollen germination, pollen tubes, rice, risk assessment, seed set, selection pressure, sexual reproduction, stigma, styles, transgenic plants, wild relatives, fertilizing, genetically engineered plants, genetically modified organisms, genetically modified plants, GEOs, GMOs, paddy, phosphinothricin, transgenic organisms, weedicides, weedkillers, pollination, grass weeds, herbicide-resistant weeds, selfing, plant fertility, ovules, weedy relatives, embryo (plant), seed productivity, plant reproduction, Aminobutyrates, Drug Resistance, Genetic Hybridization, Pollen, Reproduction, Seeds, Transgenes, Zygote, Pharmacology, Genetics, Drug Effects, Growth & Development, Physiology, reproductive compatibility, transgenic rice with bar, weedy rice
Citation: Song, X., Liu, L., Wang, Z. and Qiang, S., 2009. Potential gene flow from transgenic rice (Oryza sativa L.) to different weedy rice (Oryza sativa f. spontanea) accessions based on reproductive compatibility. Pest Management Science, 65(8), pp.862-869.
