Single-copy transgenic wheat generated through the resolution of complex integration patterns

Journal or Publishing Institution: Proceedings of the National Academy of Sciences

Study: http://www.pnas.org/content/96/20/11117.full

Author(s): Srivastava, V., Anderson, O.D. and Ow, D.W.

Article Type: Report

Record ID: 2201

Abstract: Genetic transformation of plants often results in multiple copies of the introduced DNA at a single locus. To ensure that only a single copy of a foreign gene resides in the plant genome, we used a strategy based on site-specific recombination. The transformation vector consists of a transgene flanked by recombination sites in an inverted orientation. Regardless of the number of copies integrated between the outermost transgenes, recombination between the outermost sites resolves the integrated molecules into a single copy. An example of this strategy has been demonstrated with wheat transformation, where four of four multiple-copy loci were resolved successfully into single-copy transgenes.

Keywords: Wheat Transformation, Site-Specific Recombination, Cre-lox, Transgenes, Genetic loci, DNA probes, Transgenic plants, Embryos, Nucleic acid hybridization, Segregation, Polymerase chain reaction, Triticum aestivum, genetic transformation, plasmid vectors, gene transfer, loci, genes, biolistics, alleles, nucleases, reporter genes, bilanafos, herbicide resistance, genetic recombination

Citation: Srivastava, V., Anderson, O.D. and Ow, D.W., 1999. Single-copy transgenic wheat generated through the resolution of complex integration patterns. Proceedings of the National Academy of Sciences, 96(20), pp.11117-11121.