Journal or Publishing Institution: American Journal of Botany
Study: http://www.jstor.org/stable/2445340
Author(s): Jorgensen, R.B. and Andersen, B.
Article Type: Peer Reviewed Study
Record ID: 1185
Abstract: Frequencies of spontaneous hybridization between oilseed rape (Brassica napus L.) and weedy Brassica campestris ssp. campestris L. were measured in agricultural fields. Hybrids were identified by enzyme electrophoresis, random amplified polymorphic DNA (RAPD) analysis, chromosome counting, morphology, and pollen fertility. When the two species were mixed 1:1, B. campestris produced 13% hybrid seeds and oilseed rape 9%. In two experiments with single plants of the self-incompatible B. campestris widely spaced within fields of oilseed rape, 56% and 93% hybrid seeds were produced. Analysis of a weedy population of B. campestris in oilseed rape revealed 60% hybrid seeds. Backcrossing of the hybrids to the weedy species seems to occur as supported by the finding in a natural population of two B. campestris-like plants with a marker specific to oilseed rape. The results suggest that transgenes could be dispersed from oilseed rape to B. campestris.
Keywords: Oilseeds, Hybridity, Plants, Seeds, Pollen, Genetic hybridization, Electrophoresis, Chromosomes, Crop harvesting, Plant morphology, Transgenes, Genetically Modified, Oilseed rape, Brassica napus L., Brassica campestris ssp. campestris L.
Citation: Jorgensen, R.B. and Andersen, B., 1994. Spontaneous hybridization between oilseed rape (Brassica napus) and weedy B. campestris (Brassicaceae): a risk of growing genetically modified oilseed rape. American Journal of Botany, 81(12), pp.1620-1626.
