Tea polyphenol protects mouse sertoli cells against oxidative damage and apoptosis induced by glyphosate

Journal or Publishing Institution: Department of Nutrition and Food Hygiene, Bengbu Medical College

Study: https://web.archive.org/web/20180816044621/http://aammt.tmmu.edu.cn/Upload/PaperUpLoad/c2484ed6-5d93-4fb9-a6d6-841d4eb81ed1.pdf

Author(s): Wenhong, Z., Hui, Y., Jianguo, Z. and Chengmei, J.

Article Type: Study

Record ID: 1748

Abstract: Objective: To investigate the protective effect of different doses of tea polyphenol (TP) on the mouse sertoli cells against oxidative damage and apoptosis induced by glyphosate (GLY). Methods: The oxidative damage model of primarily cultured mouse sertoli cells was established through GLY (90mg/L) incubation for 24h. Then the cells were treated with different doses of TP (10, 20, 40 and 80mg/L). Meanwhile, the control cells were incubated with normal medium. After incubation for 24h, the sertoli cells were observed for the morphological changes by inverted microscopy. Cell viability was evaluated by MTT assay. Cell cytotoxicity was measured by lactated dehydrogenase (LDH) test. Cell apoptosis was tested with TUNEL assay. The activity of super oxidase dismutase (SOD), as well as the levels of glutathione (GSH) and malondialdehyde (MDA) were also detected. Results: After the sertoli cells were treated with GLY (90mg/L), the cells became smaller, and more cast-off cells and cell debris were found. The cell viability was significantly lower than that in the normal control (P<0.05), only accounting for 47.03%. The LDH activity and the level of MDA were significantly higher (P<0.05), and the activity of SOD and the level of GSH were obviously lower than those of control group. Furthermore, the rate of cell apoptosis was (36.96±4.0)%, significantly higher than that of control group (P<0.05). Meanwhile, when sertoli cells were treated with GLY followed by different doses of TP, the states of cells were improved, especially in those treated by 80mg/LTP, and there were less cell debris found. TP inhibited the decrease of cell proliferation, SOD activity and GSH level (P < 0.05, P < 0.01). In addition, TP also inhibited the increase of MDA level and LDH activity (P < 0.05, P < 0.01). Conclusion: GLY induces apoptosis, inhibits cell proliferation, and decreases anti-oxidative ability in mouse sertoli cells. While, TP plays a protective role against these damages.

Keywords: tea polyphenol, glyphosate, mouse sertoli cells, oxidative damage, apoptosis

Citation: Wenhong, Z., Hui, Y., Jianguo, Z. and Chengmei, J., 2013. Tea polyphenol protects mouse sertoli cells against oxidative damage and apoptosis induced by glyphosate. Department of Nutrition and Food Hygiene, Bengbu Medical College, 35(19), pp.2037-2040.