Journal or Publishing Institution: Nature Biotechnology
Date of Publication: 02/07/2020
Study: https://www.nature.com/articles/s41587-019-0394-6
Author(s): Norris, A.L., Lee, S.S., Greenlees, K.J., Tadesse, D.A., Miller, M.F., Lombardi, H.A.
Article Type: Peer Reviewed Study
Abstract:
We analyzed publicly available whole genome sequencing data from cattle which were germline genome-edited to introduce polledness. Our analysis discovered the unintended heterozygous integration of the plasmid and a second copy of the repair template sequence, at the target site. Our finding underscores the importance of employing screening methods suited to reliably detect the unintended integration of plasmids and multiple template copies.
Keywords: cattle, plasmid integration, genome editing
Citation:
Norris, A.L., Lee, S.S., Greenlees, K.J., Tadesse, D.A., Miller, M.F. and Lombardi, H.A., 2020. Template plasmid integration in germline genome-edited cattle. Nature Biotechnology, 38(2), pp.163-164.
Category:
- Environmental effects
Record ID: 2503
