Journal or Publishing Institution: Nature Biotechnology
Study: https://www.biorxiv.org/content/biorxiv/early/2019/07/28/715482.full.pdf
Author(s): Norris, A.L., Lee, S.S., Greenlees, K.J., Tadesse, D.A., Miller, M.F. and Lombardi, H.A.
Article Type: Peer Reviewed Study
Abstract:
We analyzed publicly available whole genome sequencing data from cattle which were 21 germline genome-edited to introduce polledness. Our analysis discovered the unintended 22 heterozygous integration of the plasmid and a second copy of the repair template sequence, 23 at the target site. Our finding underscores the importance of employing screening methods 24 suited to reliably detect the unintended integration of plasmids and multiple template 25 copies.
Keywords: gene editing, CRISPR/Cas9, cattle
Citation:
Norris, A.L., Lee, S.S., Greenlees, K.J., Tadesse, D.A., Miller, M.F. and Lombardi, H.A., 2020. Template plasmid integration in germline genome-edited cattle. Nature Biotechnology, 38(2), pp.163-164.
Category:
- Health effects
Record ID: 2526
