The effect of Cry1Ab protein in embryo development and chemical content in Pardosa pseudoannulata

Journal or Publishing Institution: Acta Arachnologica Sinica

Study: https://web.archive.org/save/http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zxxb201401013

Author(s): Ji, Z., Bao-Yang, W., Wen-Jing, L. and Zhi, W.

Article Type: Peer Reviewed Study

Record ID: 2382

Abstract: 利用稻田蜘蛛优势种拟环纹豹蛛为研究对象,检测Cry1Ab蛋白在拟环纹豹蛛卵囊内的含量及Cry1Ab蛋白对拟环纹豹蛛胚胎发育过程中形态特征的变化、化学物质含量和卵粒内4种保护酶活性的影响.结果表明:在胚胎发育过程中,卵粒中Cry1Ab蛋白含量随发育时间延长而减少,通过对卵粒中的化学物质的测定,发现蛋白质含量水平对照组要显著高于实验组(P＜0 05),糖和脂肪含量对照组和实验组之间无显著差异.卵粒内超氧化物歧化酶(SOD)、乙酰胆碱酯酶(AchE)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)4种酶活力除了GSH-Px酶活力实验组高于对照组外,其余3种酶活力均低于对照组.通过石蜡切片和液体石蜡透明观察,实验组较对照组胚胎发育历期延长.该研究证明在胚胎发育时期Cry1Ab蛋白影响了卵粒内SOD、AchE、GSH-Px和CAT酶的活力,且Cry1Ab蛋白对拟环纹豹蛛的胚胎发育产生了一定程度的影响.

Keywords: Cry1Ab protein, embryo development, chemical content, Pardosa pseudoannulata

Citation: Ji, Z., Bao-Yang, W., Wen-Jing, L. and Zhi, W., 2014. The effect of Cry1Ab protein in embryo development and chemical content in Pardosa pseudoannulata. Acta Arachnologica Sinica, 1, pp.58-64.