Journal or Publishing Institution: Molecular Plant Microbe Interactions
Study: http://www.ncbi.nlm.nih.gov/pubmed/8274776
Author(s): Mogilner, N., Zutra, D., Gafny, R. and Bar-Joseph, M.
Article Type: Peer Reviewed Study
Record ID: 1660
Abstract: Several plant species, including tomato (Lycopersicon esculentum), Gynura aurantiaca, avocado (Persea americana), and grapefruit (Citrus paradisi) grafted on Troyer citrange (Poncirus trifoliata x C. sinensis) were “agro-infected” with Agrobacterium tumefaciens strain LBA-4404, carrying a mini-Ti plasmid with a dimeric cDNA of citrus exocortis viroid (CEVd). Extracts prepared from tissues of the agroinfected plants 38-90 days after inoculation were plated on selective media and found to contain large amounts of the engineered bacteria. These observations suggest the need for more stringent quarantine measures when handling A. tumefaciens cells harboring constructs for “agroinoculation” with plant viruses or viroids.
Keywords: Agrobacterium tumefaciens, Base Sequence, Containment of Biohazards, DNA Primers, Genetic Engineering, Genetic Vectors, Molecular Sequence Data, Plant Viruses, Plants, Viroids; Genetics, Microbiology
Citation: Mogilner, N., Zutra, D., Gafny, R. and Bar-Joseph, M., 1993. The Persistance of Engineered Agrobacterium tumefaciens in Agroinfected Plants. Molecular Plant Microbe Interactions, 6, pp.673-673.
