Journal or Publishing Institution: Bulletin Issues of Biology and Medicine
Author(s): Kulik, Y.M., Rautskiene, V.T., Obertiukh, Y.V., and Khimich, O.V.
Article Type: Peer Reviewed Study
Abstract:
Research purpose. Identify the presence of unidentified factor transgenic soybean in posterity of rats at her long-term feeding.
Object and research methods. Research conducted on white rats which were kept in the vivarium Vinnitsa National Medical University named M.I. Pirogov. Two groups of rats by 10 goals each fed a standard diet vivarium and experimental group received a standard diet additionally roasted transgenic soybeans. The experiment lasted eight generations. During this period studied the impact of transgenic soybeans in the internal organs, particularly the testes of rats that served as the basis of detection of transgenic soybean unidentified factor in the body of newborn rats. For research of the availability of rat offspring factor transgenic soy was taken on 5 rats 3 days of age with the experimental and control groups. Conducted anesthesia and euthanasia, and carcass finely crushed and placed in a heat-resistant glass cups. At 100 g mass muscle and bone tissue of the internal organs of rats was added 200 ml of distilled water, heated to boil and boiling for 1 hour. In the process of boiling occurred inactivation of biologically active substances with the transition to an aqueous solution of water-soluble fraction thermo stable proteins, lipids, minerals and low-molecular substances from the muscle-bone of the internal organs of offspring rats in the control group and, obviously, plasmid DNA Agrobacterium tumefaciens of tissue rat experimental group. After boiling broth filtered through a nylon cloth thus prepared aqueous extract from carcasses neonatal rat experimental and control group.
Research results and their discussion. On the 3rd day in a nutrient medium water extract of muscle-bone tissue of internal organs 3-day rat control group viability ciliates absent, and aqueous extract in a medium of the same age continues experimental group of rats are very active movement dark oblong adults and young ciliates, which are under active reproduction in the absence of victims. It turns out that in the offspring of female rats that over 8 generations and eating transgenic soy, biologically active compounds are present. It should be noted that these compounds, which are in the bean genetically modified soy, are able to pass through the placental barrier and remain in posterity. Literary sources indicate that the problem of biological safety of genetically modified food for humans and animal feed is extremely complex and requires a versatile study. The study was based on the absorption of dietary compounds as unidentified factor transgenic soya – alien inserts – ring Agrobacterium tumefaciens DNA from the gastrointestinal tract into the bloodstream of rats and the presence of this factor in the newborn offspring. After all, this factor should influence the physiological growth, development and metabolism newborn offspring that are not diagnosed by modern methods of research. Make conclusion about the biological hazards of transgenic soy in food for people and food of animal origin for use transgenic soybeans in feeding farm animals, which justified the promotion and propagation sustainability ciliates Tetrahymena pyriformis among water extract of muscle-bone with internal by the 3-day rat females fed transgenic soybeans compared to offspring from control animals.
Conclusions. When fed to rats over a white 8-generations of transgenic soybeans in addition to the diet of their offspring revealed the presence of unidentified factor that appears to stimulate reproduction and sustainability ciliates Tetrahymena pyriformis among water extract of the muscle-bone with internal organs 3-day rats compared to offspring from control animals. Such studies may criterion for evaluating the biological safety or health hazard people GM products of plant and animal origin.
Keywords: genetically modified soybeans, white rats, plasmid Agrobacterium tumefaciens, newborn rats, unidentified factor soybean, food, GM soy, glyphosate tolerant soy, Roundup Ready soya, rodent, feeding, GMO soybeans, rat, rodents, transgenic, genetically engineered
Citation:
Kulik, Y.M., Rautskiene, V.T., Obertiukh, Y.V., and Khimich, O.V., 2015. The presence in the offspring of rats unidentified factor transgenic soy at its feeding over several generations. Bulletin Issues of Biology and Medicine, 4 (1), pp. 105-109.
Category:
- Health effects
Record ID: 1323
