Journal or Publishing Institution: The Canadian Entomologist
Author(s): Chen, C.X., Wu, E., Yang, Y.Z., and Su, H.H.
Article Type: Peer Reviewed Study
Abstract:
In order to determine the transference of Bacillus thuringiensis Berliner (Bacillaceae) (Bt) insecticidal protein in the food chain, enzyme-linked immunosorbent assay was used to detect Bt insecticidal protein levels in transgenic Bt cotton (GK12, New variety 33B and SGK321), Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) fed on the Bt cotton varieties, and two natural enemies of S. exigua, Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae) and Microplitis pallidipes Szépligeti (Hymenoptera: Braconidae). The results showed that Bt insecticidal protein was found not only in cotton leaves, but also in the body and excrement of S. exigua and the bodies of both C. carnea and M. pallidipes. Bt toxin was detected in S. exigualarvae of all the examined instars (second, third, fourth, and fifth) that fed on transgenic cotton varieties and the Bt toxin level was the highest in the body of the second instar. In addition, the Bt toxin content in the excrement of the second instar was lower than that in the older ones. After the natural enemies C. carneaand M. pallidipes preyed/parasitised the S. exigua larvae that fed on transgenic cotton, Bt toxin was found in both the predator and parasite. This research indicates that Bt protein can be transferred through the food chain and to natural enemies of various predatory habits.
Keywords: Bt cotton, Bt insecticidal protein, GK12, 33B, SGK321, Spodoptera exigua (Hübner), Chrysoperla carnea (Stephens), Microplitis pallidipes Szépligeti, gene transfer
Citation:
Chen, C.X., Wu, E., Yang, Y.Z., and Su, H.H., 2014. The transfer of Bt insecticidal protein to higher tropic levels via a transgenic cotton, then beet armyworm (Lepidoptera: Noctuidae) and their natural enemies. The Canadian Entomologist, 146(1), 1-7.
Category:
- Environmental effects
- Pesticide use
Record ID: 366
