Journal or Publishing Institution: Journal of Inspection and Quarantine
Author(s): Ran, T., Mei, L., Lei, W., Keyong, J. and Baojie, W.
Article Type: Peer Reviewed Study
Record ID: 2029
Abstract: Tilapias (Oreochromis niloticus) were fed using transgenic soybean diets and non-transgenic soybean diets. Gastric contents, intestinal contents and faeces were sampled respectively from tilapias at 1h, 4h and 8h after feeding and different tissues of tilapias were collected at 4-, 7-week and continued hunger 2-week. DNA was extracted from these samples and the distribution of exogenous gene in different tissues was detected by nested PCR. Exogenous gene fragments were detected in DNA samples extracted from gastric contents, intestinal contents, faeces, heart, liver, stomach, intestines, ovary, spermary, brain, branchia, spleen, cholecyst, muscle of tilapias receiving transgenic soybean diets. The results demonstrated that exogenous gene of transgenic soybean couldn’t be degraded by alimentary canal of tilapia totally, and the transgene DNA can transfer to different tissues of tilapias by digestive absorption.
Keywords: Tilapias, Oreochromis Niloticus, Transgenic Soybean, Soybean Diets, DNA, Transgene DNA, Digestive Absorption
Citation: Ran, T., Mei, L., Lei, W., Keyong, J. and Baojie, W., 2009. Tissues Studies of Tilapias fed with Transgenic Soybean Feedstuff. Journal of Inspection and Quarantine, 2, p.009.
