Journal or Publishing Institution: Journal of Agricultural and Food Chemistry
Study: http://www.ncbi.nlm.nih.gov/pubmed/16277398
Author(s): Prescott, V.E., Campbell, P.M., Moore, A., Mattes, J., Rothenberg, M.E., Foster, P.S., Higgins, T.J.V. and Hogan, S.P.
Article Type: Peer Reviewed Study
Record ID: 1947
Abstract: The development of modern gene technologies allows for the expression of recombinant proteins in non-native hosts. Diversity in translational and post-translational modification pathways between species could potentially lead to discrete changes in the molecular architecture of the expressed protein and subsequent cellular function and antigenicity. Here, we show that transgenic expression of a plant protein (alpha-amylase inhibitor-1 from the common bean (Phaseolus vulgaris L. cv. Tendergreen)) in a non-native host (transgenic pea (Pisum sativum L.)) led to the synthesis of a structurally modified form of this inhibitor. Employing models of inflammation, we demonstrated in mice that consumption of the modified alphaAI and not the native form predisposed to antigen-specific CD4+ Th2-type inflammation. Furthermore, consumption of the modified alphaAI concurrently with other heterogeneous proteins promoted immunological cross priming, which then elicited specific immunoreactivity of these proteins. Thus, transgenic expression of non-native proteins in plants may lead to the synthesis of structural variants possessing altered immunogenicity.
Keywords: α-Amylase inhibitor; transgenic plant; animal model; Th2 inflammation; mass spectrophotometry; Animals, Diet, Gene Expression, Immunity, Inflammation, Mice, Molecular Sequence Data, Peas, Plant Lectins, Genetically Modified Plants, Th2 Cells, immunology, genetics, drug effects; Phaseolus vulgaris, beans, enzyme inhibitors, alpha-amylase, transgenes, gene expression, Pisum sativum, transgenic plants, peas, protein structure, immune response, food intake, risk assessment, genetically modified foods, human food chain, lungs, eosinophils, mice
Citation: Prescott, V.E., Campbell, P.M., Moore, A., Mattes, J., Rothenberg, M.E., Foster, P.S., Higgins, T.J.V. and Hogan, S.P., 2005. Transgenic expression of bean α-amylase inhibitor in peas results in altered structure and immunogenicity. Journal of Agricultural and Food Chemistry, 53(23), pp.9023-9030.
