Journal or Publishing Institution: Journal of Agricultural and Food Chemistry
Study: http://www.ncbi.nlm.nih.gov/pubmed/20050687
Author(s): Jiao, Z., Si, X.X., Li, G.K., Zhang, Z.M. and Xu, X.P.
Article Type: Peer Reviewed Study
Record ID: 1158
Abstract: Unintended compositional changes in transgenic rice seeds were studied by near-infrared reflectance, GC-MS, HPLC, and ICP-AES coupled with chemometrics strategies. Three kinds of transgenic rice with resistance to fungal diseases or insect pests were comparatively studied with the nontransgenic counterparts in terms of key nutrients such as protein, amino acids, fatty acids, vitamins, elements, and antinutrient phytic acid recommended by the Organization for Economic Co-operation and Development (OECD). The compositional profiles were discriminated by chemometrics methods, and the discriminatory compounds were protein, three amino acids, two fatty acids, two vitamins, and several elements. Significance of differences for these compounds was proved by analysis of variance, and the variation extent ranged from 20 to 74% for amino acids, from 19 to 38% for fatty acids, from 25 to 57% for vitamins, from 20 to 50% for elements, and 25% for protein, whereas phytic acid content did not change significantly. The unintended compositional alterations as well as unintended change of physical characteristic in transgenic rice compared with nontransgenic rice might be related to the genetic transformation, the effect of which needs to be elucidated by additional studies.
Keywords: chemometrics methods; compositional changes; discrimination; Oryza sativa L.; Transgenic rice seeds, Oryza, Oryza sativa, plants, amino acids, analytical methods, fatty acids, GC-MS, genetically engineered organisms, genotypes, HPLC, mineral content, nutritive value, phytic acid, protein content, rice, transgenic plants, vitamins, analytical techniques, gas chromatography-mass spectrometry, genetically engineered plants, genetically modified organisms, genetically modified plants, GEOs, GMOs, high performance liquid chromatography, inositol hexaphosphate, nutritional value, paddy, phytate, quality for nutrition, transgenic organisms, Poaceae, Poales, commelinids, monocotyledons, angiosperms, Spermatophyta, plants, eukaryotes, Oryza
Citation: Jiao, Z., Si, X.X., Li, G.K., Zhang, Z.M. and Xu, X.P., 2010. Unintended compositional changes in transgenic rice seeds (Oryza sativa L.) studied by spectral and chromatographic analysis coupled with chemometrics methods. Journal of Agricultural and Food Chemistry, 58(3), pp.1746-1754.
