GMO Research
Abstract: The 35S cauliflower mosaic virus (CaMV) promoter is commonly used to drive transgene expression in the genetically engineered (GE) crop plants that have been commercialized so far. Whether, and how far, the 35S promoter might be active in mammalian cells has been scientifically unsettled and controversial. Very recently it was established that the 35S …
Abstract: The long-term persistence and activity of a naked plasmid DNA (pGL3-35S) containing a luc gene (reporter gene) controlled by a plant 35S CaMV promoter was studied in Atlantic salmon (Salmo salar L.) after injection. Atlantic salmon (mean weight 70 grams) were injected intramuscularly with 100 μg of plasmid DNA. Blood, different tissues and organs …
Text: We would like to address the suggestion of Matzke et al. (1) and others that pararetroviral promoters such as the CaMV 35S are not exotic to plant genomes as they already contain many integrated pararetroviral sequences. The crucial question is whether the CaMV 35S promoter in transgenic constructs poses special risks. We would like …
To the editor: In your account (January 2000) of our prepublication manuscript1, you quote the criticisms but ignore completely our full rebuttal, which was posted on the web last November2. Our manuscript3 reviews and synthesizes the scientific literature on the 35S promoter of the cauliflower mosaic virus (CaMV), used to give constitutive overexpression of transgenes …
Abstract: The expression of foreign genes in pollen may pose potential problems in the field release of transgenic plants, since pollen represents a route whereby foreign genes and their products may escape into the wider environment. The possible risks posed by cross-hybridization with wild relatives have been extensively explored, but problems that may arise due …
Abstract: Multiple variants of the Cauliflower mosaic virus 35S promoter (P35S) are used to drive the expression of transgenes in genetically modified plants, for both research purposes and commercial applications. The genetic organization of the densely packed genome of this virus results in sequence overlap between P35S and viral gene VI, encoding the multifunctional P6 …
Abstract: Genetically modified (GM) food crops are considered to have the potential of providing food security especially in developing countries. Scientists have raised concern over the hazards associated with the consumption of genetically modified organisms (GMOs). One of these hazards, which have great controversy reports, is the possible horizontal gene transfer from GM-food or feed …
Abstract: Activation tagging using T-DNA vectors that contain multimerized transcriptional enhancers from the cauliflower mosaic virus (CaMV) 35S gene has been applied to Arabidopsis plants. New activation-tagging vectors that confer resistance to the antibiotic kanamycin or the herbicide glufosinate have been used to generate several tens of thousands of transformed plants. From these, over 30 …
Abstract: Qualitative and quantitative DNA-based methods were applied to detect genetically modified foods in samples from markets in the Kingdom of Saudi Arabia. Two hundred samples were collected from Al-Qassim, Riyadh, and Mahdina in 2009 and 2010. GMOScreen 35S and NOS test kits for the detection of genetically modified organism varieties in samples were used. …
Abstract: Soil properties, microbial communities, and enzyme activities were studied in soil planted with transgenic or nontransgenic papaya under field conditions. The transgenic papaya contained a replicase (RP) mutant gene of the papaya ringspot virus (PRSV), which conferred resistance to the virus, the neomycin phosphotransferase II (NPT II) marker gene, which conferred Km resistance, and …
Abstract: Samples of rice from Mexican and USA retail stores were analyzed for the presence of transgenic (GM) events using real-time PCR. In screening for the CaMV35S promoter sequence (35SP), positive results were found in 49 and 35% of the Mexican and American samples, respectively. In further investigations in Mexican samples, 43% were positive for …
Abstract: Different transgenic plants of Brassica napus, Brassica nigra, Datura innoxia and Vicia narbonensis expressing the hph gene under the control of the 35s promoter were co-cultivated with mycelial material of Aspergillus niger in microcosms under sterile conditions. A significantly higher number of hygromycin B-resistant colonies of re-isolated fungi was obtained if compared with co-cultures …
Abstract: The soybean is a typical legume which is used to elaborate several foods around the world. Ten imported soybean seed samples were collected and planted to identify possible genetic modifications. DNA was isolated from 20 days old seedlings. After that, PCR was carried out using the 35S, RR and cry1AB/1AS primer pairs. Nine soybean …
Abstract: The in vivo and post mortem performance and serum immunoglobulin G (IgG) concentration in kids born from goats fed conventional (group C) or genetically modified (group T) soybean meal were evaluated. The goat colostrum quality, in terms of chemical composition, as well as immunoglobulin concentration, and the presence of feed DNA fragments were also …
Abstract: The presence of DNA fragments in tissues from rabbits given genetically modified (GM) soya-bean meal (solvent extracted) was investigated by using the polymerase chain reaction (PCR) approach. Moreover, the possible effects on cell metabolism were evaluated by determination of several specific enzymes in serum, heart, skeletal muscle, liver and kidney. The chloroplast sequence for …
Abstract: Most traits introduced into genetically engineered crops result from the expression of new proteins. As the first step toward assessing the allergenic potential of genetically modified organism (GMO) food, immunologic and physicochemical characterizations are needed. We prepared crude extract from GMO soybeans, wild soybeans, curd, and soy milk and then performed sodium dodecyl sulfate-polyacrylamide …
Abstract: The article is devoted to the introduction of transgenic products into the consumer market. The impact of the genetically modified potatoes on the cytological and morphological parameters of mice was investigated. Transgenic potato plants were produced by agrobacterium-mediated transformation using genetic constructions 35S-proSmAMP with marker gene npt11 of the resistance to the kanamycin. An increase in hearts …
Abstract: Milk has many nutrients that make it an ideal food all over the world and Iraq is one of the developing countries who imports milk to supply the people with this important food.And because most milk producer in the world depend on genetically modified (GM) feed to fed their animals, so these GM DNA …
Abstract: We have shown previously that gene VI of cauliflower mosaic virus (CaMV) strain D4 governs systemic infection of Nicotiana bigelovii and that transgenic N. bigelovii expressing the D4 gene VI product can complement at least one CaMV isolate for long-distance transport. We have now found that DNA of two other isolates of CaMV recombine …
Abstract: The presence of DNA fragments in blood and milk from goats fed conventional (control) or Roundup Ready® soybean meal solvent extracted (s.e.; treated) was investigated by using a polymerase chain reaction approach. The same investigation was carried out on blood, skeletal muscle and organs from kids of both groups fed only dams’ milk until …
Abstract: Nowadays genetically modified organisms are commonly included in various areas of modern economy and especially in food industry. Genetically modified crops are used not only as a cheap feed for cattle, but also in food consumed by people. However, the impact of GMOs on animals and humans is still poorly understood. Because the 35S …
Abstract: A green fluorescent protein (GFP) transgene under the control of the 35S cauliflower mosaic virus (CaMV) promoter was introduced by Agrobacterium-mediated transformation into Nicotiana benthamiana to generate fourteen transgenic lines. Homozygous lines that contained one or two copies of the transgene showed great variation of GFP expression under ultraviolet (UV) light, which allowed classification …
Abstract: Concerns have been raised over the spread of transgenic DNA by horizontal gene transfer. One main factor determining the success of horizontal gene transfer is its tendency to recombine. This paper examines the safety implication of recent revelations on the recombination hotspot of the cauliflower mosaic viral (CaMV) promoter, which is in practically all …
Abstract: Rice is one of the staple foods of the Iraqi population; therefore large amounts of rice cultivated and imported in Iraq. Because of increasing the production of GM crops especially rice crop, it was necessary to investigate if there is any genetically modified rice (GM rice) in Baghdad markets. Conventional PCR and Real-Time PCR …
